中华放射医学与防护杂志

苟巧,张伟,王春燕,苏旭.α粒子诱发BEP2D细胞恶性转化中抗氧化能力和辐射敏感性研究[J].中华放射医学与防护杂志,2011,31(1):1-5

α粒子诱发BEP2D细胞恶性转化中抗氧化能力和辐射敏感性研究

Antioxidant ability and radiosensitivity in malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle irradiation

投稿时间：2010-06-21

DOI：10.3760/cma.j.issn.0254-5098.2011.01.001

中文关键词: 人支气管上皮细胞 α粒子抗氧化能力辐射敏感性癌变

英文关键词:Human bronchial epithelial cell line α-particle Antioxidant ability Radio-sensitivity Carcinogenesis

基金项目:国家自然科学基金(81000862);中国科学技术部社会公益研究专项(2005DIB1J087);卫生行业科研专项(200802018)

作者	单位
苟巧	100088 北京,中国疾病预防控制中心辐射防护与核安全医学所
张伟	100088 北京,中国疾病预防控制中心辐射防护与核安全医学所
王春燕	100088 北京,中国疾病预防控制中心辐射防护与核安全医学所
苏旭	100088 北京,中国疾病预防控制中心辐射防护与核安全医学所

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中文摘要:

目的研究α粒子诱发人支气管上皮细胞BEP2D恶性转化中细胞的抗氧化能力和辐射敏感性变化。方法运用谷胱甘肽过氧化物酶(GPX)、过氧化氢酶(CAT)和谷胱甘肽(GSH)试剂盒分别检测永生化人支气管上皮细胞BEP2D、α粒子作用BEP2D后第21代细胞RH21和α粒子作用BEP2D细胞后形成的恶性转化细胞BERP35T-1内抗氧化酶GPX和CAT的活性以及抗氧化剂GSH的含量;四甲基偶氮唑盐比色(MTT)法检测0、30、60、90、120和150 μmol/L H₂O₂作用BEP2D、RH21和BERP35T-1细胞96 h后,细胞增殖率的差异;0、2、4和8 Gy γ射线照射BEP2D、RH21和BERP35T-1细胞7~9 d后,用细胞克隆计数和MTT法检测细胞增殖率和存活分数的变化。结果 RH21和BERP35T-1细胞中GPX酶活力均低于BEP2D细胞(t=5.75和7.65,P<0.05)。BEP2D细胞中CAT酶活力是RH21细胞的2.64倍,是BERP35T-1细胞的5.76倍。RH21细胞中GSH含量与BEP2D细胞比较,差异无统计学意义;恶性转化细胞BERP35T-1内GSH含量则明显低于BEP2D细胞(t=7.76,P<0.05)。与BEP2D细胞相比,60、90、120和150 μmol/L H₂O₂作用后RH21细胞的增殖率降低(t=29.90~84.68,P<0.01),30、60、90、120和150 μmol/L H₂O₂作用后BERP35T-1细胞的增殖率降低(t=10.37~58.36,P<0.01)。4和8 Gy γ射线照射后,RH21细胞的增殖率和存活分数均显著低于BEP2D细胞(t=6.33~45.00,P<0.05);2、4和8 Gy γ射线照射后,BERP35T-1细胞的增殖率和存活分数较BEP2D细胞明显降低(t= 5.87~ 34.17,P<0.05)。结论 α粒子诱发人支气管上皮细胞BEP2D恶性转化过程中,细胞的抗氧化能力降低、辐射敏感性增强。抗氧化系统功能减弱可能是α粒子诱发BEP2D细胞恶性转化和辐射敏感性增强的机制之一。

英文摘要:

Objective To investigate the antioxidant ability and radiosensitivity in the malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle exposure. Methods Glutathione Peroxidase (GPX), Catalase (CAT) and Glutathione (GSH) assay kits were employed to detect GPX and CAT enzyme abilities and the levels of GSH in BEP2D, RH21 (passage 21 of α-particle-irradiated BEP2D cells), and BERP35T-1 cells (derived from nude mice bearing malignant transformed cells generated from cells of passage 35 of α-particle-irradiated BEP2D cells). MTT assay were used to test the growth rate of BEP2D, RH21 and BERP35T-1 cells treated with 0, 30, 60, 90, 120, and 150 μmol/L H₂O₂. Colony-forming test and MTT assay were used to examine the cell survival fraction and the growth rate of BEP2D, RH21 and BERP35T-1 cells exposed to 0, 2, 4, and 8 Gy of γ-rays, respectively. Results GPX and CAT enzyme activities in RH21 and BERP35T-1 cells were obviously lower than in BEP2D(t=5.75-67.92,P<0.05). CAT enzyme activity in BERP35T-1 was lower than that in RH21 cells(t=22.25,P<0.01). Compared to BEP2D cells, decreased level of GSH was detected in BERP35T-1 cells(t=7.76,P<0.05), but there was no change in RH21. After treatment with 30, 60, 90, 120, and 150 μmol/L H₂O₂, the growth rates of BEP2D were all higher than those of BERP35T-1 cells(t=10.37-58.36,P<0.01). Meanwhile, the growth rates of BEP2D were all higher than those of RH21 cells after treatment with 60, 90, 120, and 150 μmol/L H₂O₂(t= 29.90-84.68,P<0.01). In addition, compared to BEP2D cells, decreased cell survival fraction and growth rate of BERP35T-1 cells were observed after irradiation with 2, 4, and 8 Gy of γ-rays(t=5.87-34.17,P<0.05). The cell survival fraction and growth rate of RH21 were all lower than those of BEP2D cells at 4 and 8 Gy post-irradition(t= 6.33- 45.00,P<0.05). Conclusion The function of antioxidant system decreased in the α-particle-induced transformed cells, which could contribute to the acceleration of cellular malignant transforming process and radiosensitivity.

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