| 罗鸣,伍钢,范丽,张瑞光,任精华,董继华,董晓荣.Corilagin对小鼠小胶质细胞株照射后炎性反应因子表达的抑制作用[J].中华放射医学与防护杂志,2010,30(6):682-686 |
| Corilagin对小鼠小胶质细胞株照射后炎性反应因子表达的抑制作用 |
| Inhibitory effect of Corilagin on the inflammatory response of irradiated microglia BV-2 cells |
| 投稿时间：2010-02-09 |
| DOI： |
| 中文关键词: Corilagin 小胶质细胞 NF-κB 放射性脑损伤 |
| 英文关键词:Corilagin Microglia BV-2 NF-κB Radiation encephlopathy |
| 基金项目:国家自然科学基金(30800283) |
|
| 摘要点击次数: 2923 |
| 全文下载次数: 2100 |
| 中文摘要: |
| 目的 研究抗炎药物Corilagin抑制放射引起小胶质细胞(BV-2)炎性反应的作用及其防护的分子机制。方法 细胞抑制实验(MTT)检测Corilagin对BV-2细胞的抑制率;Corilagin预处理小胶质细胞,12 h后,以0和32 Gy 2个放射剂量照射BV-2细胞,Real-time PCR检测小胶质细胞照射后不同时间点炎性反应因子IL-1β、TNF-α表达水平;硝酸还原酶法检测细胞上清液中一氧化氮(NO)的含量;Western blot检测各组细胞核转录因子NF-κB p65蛋白表达;激光共聚焦显微镜观察各组细胞标志物Iba-1的表达、NF-κB p65核转位及Nemo的表达。结果 1～10 μg/ml浓度范围内Corilagin对BV-2细胞增殖几乎无影响;照射后小胶质细胞表面标志物Iba-1表达明显,提示小胶质细胞激活,且炎性反应因子NO、TNF-α、IL-1β表达上调,而Corilagin(5μg/ml)可以显著抑制上述炎性反应因子的表达(tIL-1β=6.341, tTNF-α=3.411, tNO=3.134, P <0.05);Corilagin可抑制NF-κB活化蛋白Nemo,并显著抑制NF-κB p65的转位。结论 Corilagin可能通过NF-κB信号转导途径抑制照射后小胶质细胞的活化,从而下调炎性反应因子表达,保护神经元。 |
| 英文摘要: |
| Objective To explore the inhibitory effects of Corilagin on the production of pro-inflammatory cytokines in microglia induced by radiation.Methods The cytotoxicity of Corilagin was measured by MTT assay. Microglia BV-2 cells were irradiated 0 or 32 Gy after pretreated with Corilagin for 12 hours. Realtime-PCR was used to detect the mRNA levels of inflammatory cytokines, such as IL-1β, TNF-α on several time-points. The content of nitric oxide (NO) was determined with nitrate reductase method. The translocation of NF-κB was measured by Western blot and immunocytochemical stain. Confocal microscopy was used to observe the expression of Iba-1 and Nemo. Results No cytotoxicity was detected on BV-2 cells with 1-10 μg/ml Corilagin. Iba-1 expression in microglia cells was activated by irradiation, the expression levels of inflammatory cytokines, such as IL-1β, TNF-α and NO were also elevated. Whereas, the production of IL-1β, TNF-α in activated microglia cells was significantly inhibited with 5 μg/mL corilagin (tIL-1β=6.341, tTNF-α=3.411, tNO=3.134, P <0.05). Corilagin significantly inhibited the expression of Nemo and the translocation of NF-κB p65. Conclusion Corilagin could inhibit the activation of irradiated microglia cells and down-regulate the expression of inflammatory cytokines, via inhibition of the NF-κB signaling pathway. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|