| 李玉文,封江彬,陆雪,范莉,陈德清,刘青杰.60Coγ射线诱导人淋巴细胞线粒体COX基因表达改变的研究[J].中华放射医学与防护杂志,2010,30(6):677-681 |
| 60Coγ射线诱导人淋巴细胞线粒体COX基因表达改变的研究 |
| Expression changes of in human mitochondrial COX genes in human lymphocytes after exposed by 60Co γ-rays |
| 投稿时间：2009-12-17 |
| DOI： |
| 中文关键词: 60Co γ射线 人淋巴细胞株 线粒体 COX 基因表达 |
| 英文关键词:60Co γ-rays Human lymphoblastoid cell line Mitochondrion COX Gene expression |
| 基金项目:国家自然科学基金(30870749, 30570551) |
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| 摘要点击次数: 2749 |
| 全文下载次数: 1949 |
| 中文摘要: |
| 目的 探讨电离辐射诱导人淋巴细胞线粒体COXⅠ、COXⅡ和COXⅢ基因表达的改变。方法 用1、3、5、8和10 Gy 60Co γ射线分别照射指数增长期的人淋巴细胞永生化细胞株,8 h后用逆转录PCR(RT-PCR)和实时荧光定量PCR(Real-time PCR)方法检测COXⅠ、COXⅡ和COXⅢ基因在mRNA水平上的表达;流式细胞术检测其在蛋白水平上的表达;比色法检测COX活性,来分析辐射诱导线粒体COXⅠ、COXⅡ和COXⅢ基因表达改变的量效关系。同时,以5 Gy γ射线照射人淋巴细胞,分别于照后不同时间(0.5、4、8、12、24、48和72 h),同样通过上述指标来分析3种线粒体基因的表达变化,观察其时效关系。 结果 在mRNA水平上,线粒体COXⅠ、COXⅡ和COXⅢ基因表达总体上调。COXⅠ和COXⅢ基因在0～3 Gy剂量范围内具有量效关系,而COXⅡ基因在0～8 Gy剂量范围内具有量效关系(FCOXⅠ=116.62,FCOXⅡ=17.89,FCOXⅢ=8.20,P<0.05);5 Gy 照后4 h左右COXⅡ和COXⅢ基因表达上调最显著,而COXⅠ基因持续低表达(F COXⅠ=31.99,FCOXⅡ=19.47,FCOXⅢ=20.64,P<0.05)。在蛋白水平上,不同剂量照射后,COXⅠ和COXⅡ蛋白表达先下调后上调,COXⅢ蛋白表达下调(FCOXⅠ=16.96,FCOXⅡ=32.5,FCOXⅢ=6.51,P<0.05);5 Gy照后4 h,COXⅠ蛋白表达明显增强,达到8 h后出现COXⅡ蛋白表达显著上调,而COXⅢ蛋白表达普遍下调(FCOXⅠ=14.68,FCOXⅡ=17.18,FCOXⅢ=2.52,P<0.05)。此外, 受照后COX活性普遍降低。结论 电离辐射可以诱导人淋巴细胞线粒体COXⅠ、COXⅡ和COXⅢ基因表达的改变,基因表达总体增强的同时,COX活性普遍降低。 |
| 英文摘要: |
| Objective To explore the changes of human mitochondrial COXⅠ, COXⅡ and COXⅢ genes expression induced by ionizing irradiation.Methods Changes of human COX genes expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines, which were exposed to 1-10 Gy60Co γ-rays. The protein levels were detected by flow cytometry and the COX activity was measured by colorimetry. The dose-effect relationships between the expression changes of the genes and the doses were established. The changes of these genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect.Results The expression of 3 genes at mRNA level was up-regulated. A good dose-effect relationship was showed for COXⅠand COXⅢ at dose range of 0-3 Gy and 0-8 Gy for COXⅡ (F COXⅠ=116.62, FCOXⅡ=17.89, FCOXⅢ=8.20, P<0.05). For the time-effect after irradiation, the gene expression levels of COXⅡ and COXⅢ genes were up-regulated and the peak change occurred at 4 h after irradiation. For COXⅠgene, the mRNA expression levels were down-regulated during 0.5-72 h (FCOXⅠ=31.99, FCOXⅡ=19.47, FCOXⅢ=20.64, P<0.05). At the protein level, the levels of COXⅠ and COXⅡ were lowered in lower doses and enhanced in higher doses, and the levels of COX Ⅲ were decreased at all dose levels (FCOXⅠ=16.96, FCOXⅡ=32.5, FCOXⅢ=6.51, P<0.05). The protein levels of COXⅠ and COXⅡ were enhanced during 4-72 h and 8-72 h respectively after 5 Gy irradiation (FCOXⅠ=14.68, FCOXⅡ=17.18, FCOXⅢ=2.52, P<0.05). The activities of COX were lowered at different dose levels and different time-points.Conclusions Ionizing radiation might induce the changes in mitochondrial COXⅠ, COXⅡ and COXⅢ gene expression, and lead to the reduction of the COX activities. |
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