| 郭玲,吕超,何英杰,丁振华.miR-21在UVB照射的人角质形成细胞和人表皮鳞癌细胞中的差异表达[J].中华放射医学与防护杂志,2010,30(6):674-676 |
| miR-21在UVB照射的人角质形成细胞和人表皮鳞癌细胞中的差异表达 |
| Differential expression of miR-21 in UVB irradiated HaCaT cells and A431 cells |
| 投稿时间：2009-12-09 |
| DOI： |
| 中文关键词: miR-21 HaCaT A431 紫外线B |
| 英文关键词:miR-21 HaCaT A431 UVB |
| 基金项目:国家自然科学基金(30970673);广东省自然科学基金(9151022501000013) |
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| 中文摘要: |
| 目的 方法 研究miR-21在紫外线B(UVB)照射的人角质形成细胞HaCaT和人表皮鳞癌细胞A431中的差异表达。方法 50 J/m2 UVB照射HaCaT细胞和A431细胞后,通过实时荧光定量RT-PCR检验miR-21的表达水平;利用在线数据库PicTar等预测靶基因并用Gostat软件对靶基因基因进行功能分类。结果 miR-21在表皮鳞状细胞癌中的表达是正常角质形成细胞的4倍多;miR-21在经UVB照射后HaCaT细胞中,在2～4 h内,其表达水平是升高的,在随后的8 h,其表达水平与对照相比降低了2倍,到12 h后表达水平就不再变化;在照射后的A431细胞中,miR-21的表达水平没有明显改变。在对其靶基因预测后用Gostat进行功能分类发现,PIK3R1、BCL2、E2F3等基因参与细胞的分化及细胞的周期进程。结论 miR-21可能参与表皮鳞状细胞癌的致病机理,并可能在UVB诱导的损伤机制中发挥作用。 |
| 英文摘要: |
| Objective To study the expression level of miR-21 in UVB irradiated HaCaT cells and A431 cells. Methods Real-time qPCR was used to examine the expression level of miR-21 in HaCaT cells and A431 cells after 50 J/m2 UVB radiation. The possible target genes were predicted by PicTar and performed function categories with Gostat analysis.Results Compared with the HaCaT cells, miR-21 the expression level in A431 cells increased over 4 times. At 2h and 4h after UVB irradiation, the expression level of miR-21 in HaCaT cells were up regulated, and it lowered 2 times at 8 h compared with the control. There was no further change in the expression level of miR-21 after 12 h. While miR-21 expression levels in A431 cells were not changed signifcantly. The results of target prediction and Gostat analysis suggested that PIK3R1, BCL2 and E2F3 were involved in the cell differentiation and cell process. Conclusion miR-21 possibly involved in the pathogenesis of epidermal squamous cell carcinoma and the mechanism of UVB-induced injury. |
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