| 潘麓羽,曹建平,纪蓉,封阳,周媛媛,彭晓梅,刘杨,陈遐林,崔丹,朱巍,樊赛军.蒿甲醚对人鼻咽癌细胞CNE-1放射敏感性的影响及其机制研究[J].中华放射医学与防护杂志,2010,30(5):550-553 |
| 蒿甲醚对人鼻咽癌细胞CNE-1放射敏感性的影响及其机制研究 |
| Effect of artemether on radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1 |
| 投稿时间：2009-11-28 |
| DOI： |
| 中文关键词: 蒿甲醚 鼻咽癌 放射敏感性 |
| 英文关键词:Artemether Nasopharyngeal neoplasms Radiosensitivity |
| 基金项目:国家自然科学基金(30670632);教育部长江学者创新团队项目(IRT0849) |
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| 中文摘要: |
| 目的 研究蒿甲醚(artemether)对人鼻咽癌CNE-1细胞放射敏感性的影响及其作用机理。方法 用MTT法检测蒿甲醚对CNE-1细胞生长的抑制效应;克隆形成法检测蒿甲醚对CNE-1细胞放射敏感性的影响;流式细胞仪检测不同处理因素对CNE-1细胞的细胞周期的影响;Western blot分析蒿甲醚对电离辐射诱导的CNE-1细胞中的细胞周期调控蛋白Cyclin B1和Wee1表达水平的影响。结果 蒿甲醚可抑制CNE-1细胞的增殖,且药物浓度和作用时间相关;20 μmol/L蒿甲醚对CNE-1细胞有放射增敏作用,其放射增敏作用随作用时间的延长而延长,作用24 h 后增敏作用最强,放射增敏比(SER)为1.481。流式细胞仪分析表明,单纯60Co γ射线照射后G2/M期细胞比例上升,而G0/G1期细胞比例下降;药物和射线联合作用后,与单纯照射组相比,G0/G1期细胞比例上升(t=4.59,P<0.05),G2/M期细胞比例下降(t=10.60,P<0.05)。蒿甲醚可降低照射后CNE-1细胞内Wee1蛋白表达水平,提高Cyclin B1蛋白表达水平。结论 低浓度蒿甲醚对CNE-1 细胞有放射增敏作用;蒿甲醚通过改变细胞周期调控蛋白Cyclin B1和 Wee1表达水平,诱导G2/M 期阻滞而发挥放射增敏作用。 |
| 英文摘要: |
| Objective To evaluate the effect of artemether on the cell cycle and the radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1. Methods Cell growth inhibition was assessed with MTT. The method of colony-forming was used to detect the radiation sensitivity. Cell cycle distribution was analyzed by using flow cytometry. The protein expressions of clyclin B1 and Wee1 were detected by using Western blot. Results The growth of CNE-1 cells was inhibited in a dose-dependent manner. The concentration of 20 μmol/L artemether had radiosensitive effect on CNE-1 cells at 24 h after administration, and SER was 1.481. When CNE-1 cell was irradiated, the G2/M cells increased (t=4.59, P<0.05). After exposure to combination of artemether and irradiation, the G2/M cells were decreased (t=10.60, P<0.05). Western blot showed that artemether increased the level of cyclin B1 expression and inhibited the level of Wee1 expression. Conclusions The noncytotoxic concentration of artemether could enhance radiosensitization of CNE-1 cells. The radiosensitivity enhancement of artemether might depend on the exposure time. The effect is most obvious when radiation is delivered 24 h after expose to artemetherr. The radiosensitizing effect could be related to apoptosis. |
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