| 常公民,彭瑞云,高亚兵,王水明,李扬,徐新萍,王丽峰,董霁,赵黎.重组人白细胞介素-11和姜黄素对中子照射致小鼠肠道损伤的治疗作用[J].中华放射医学与防护杂志,2010,30(5):531-534 |
| 重组人白细胞介素-11和姜黄素对中子照射致小鼠肠道损伤的治疗作用 |
| Therapeutic effect of recombinant human interleukin-11 and curcumin on jejunal damage in mice after neutron irradiation |
| 投稿时间：2009-11-13 |
| DOI： |
| 中文关键词: 中子 小鼠 空肠 重组人白细胞介素-11 姜黄素 |
| 英文关键词:neutron mouse intestinal rhIL-11 curcumin |
| 基金项目:国家自然科学基金(30870735) |
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| 中文摘要: |
| 目的 探讨重组人白细胞介素-11(rhIL-11)和姜黄素对中子照射致小鼠肠道损伤的治疗作用。方法 选取二级雄性BALB/c小鼠140只,随机分为健康对照组(20只)、单纯照射组(60只)、IL-11治疗组(30只)和姜黄素治疗组(30只)。单纯照射组小鼠采用3 Gy中子全身均匀照射,两个治疗组于照射后即日分别腹腔注射500μg·kg-1·d-1的rhIL-11或200 mg·kg-1·d-1的姜黄素,每天1次,连续给药5d。观察各组动物的死亡率。照射后6 h、1、3和5 d分别处死健康对照组和单纯照射组动物,3和5 d分别处死两个治疗组动物。取空肠标本,用HE深色、核仁组成区嗜银蛋白染色、Feulgen氏染色和图像死亡分析等,检测空肠病理形态变化、空肠上皮细胞核内嗜银蛋白含量和DNA含量变化。结果 照后5d,单纯照射组小鼠全部死亡,rhIL-11治疗组存活剩余2只,姜黄素治疗组存活剩余3只。3Gy中子照射后6 h～3 d内,空肠黏膜大面积坏死脱落,3d偶见隐窝细胞再生,5d见较多新生隐窝;IL-11治疗组在照后3d隐窝再生较明显,5d见大量新生绒毛;姜黄素治疗组在照后3d隐窝有部分再生,5d可见新生绒毛,排列结构较IL-11治疗组紊乱,绒毛高度较IL-11治疗组略低。照后5d,两个治疗组小鼠空肠上皮细胞AgNOR含量和DNA含量均高于单纯照射组(F＝0.015～0.035,P<0.05)。结论 3 Gy中子照射引起小鼠空肠明显损伤,rhIL-11和姜黄素治疗可减轻损伤并促进肠道上皮再生修复,对中子辐射肠上皮损伤具有保护作用。 |
| 英文摘要: |
| Objective To explore the therapeutic effect of recombinant human interleukin(rhIL-11) and curcumin on jejunaldamage in mice after neutron irradiation. Methods 140 male BALB/c mice were randomly divided into 4 groups:20 mice in healthy control group, 60 mice in mere irradiation group, 30 mice in IL-11 treatment group and 30 mice in curcumin treatment group. The mere irradiation group mice were wholly exposed to 3 Gy neutron irradiation. The treatment groups mice were intraperitoneally injected with rhIL-11 at the dosage of 500 μg·kg-1·d-1 and ourcumin of 200 mg·kg-1·d-1 through enterocoelia once a day for 5 d after irradiation. The mortality of the mice were observed.The mice in the control and mere irradiation groups were killed 6 h, 1, 3, and 6 dpost-irradiation, respectively, and the mice of the 2 treatment groups were killed 3 and 6 d post-irradiation, respectively and the samples of jujunum were colleted. HE staining, argyrophilic of nucleaolar organizer regions staining, Feulgen staining, and image analysis were used to observe the pathology and levels of argyrophilic proteins and DNA. Results The mice in the mere irradiation group all died at 5 d post-irradiation, while 2 mice in the IL-11 treatment group and 3 in the curcumin group survived. Large area necrosis and exfoliation were found in the intestinal epithelial mucosa of the mere irradiated group mice since 6 h to 3 d after irradiation. Crypt cell regeneration was seen occasionally found 3 days later and much more 5 days later. Crypt cell regeneration was obviously found in the intestinal epithelial mucosa and lots of new villi were observed 5 d after irradiation in both treatment groups, however, the amounts of crypt cells and new villi of the curcumin treatment group were less than those of the IL-11 treatment group. The contents of AgNOR and DNA in the intestinal epithelial cells 5 days after irradiation of the 2 treatment groups were all significantly higher than those of the mere irradiation group (F=0.015-0.035,all P<0.05) but without significant differences between them. Conclusions Jejunal damage in mice could be induced after 3 Gy neutron irradiation. rhIL-11 and curcumin might reduce the damage and promote the regeneration and repair of the intestinal epithelium. |
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