| 王晶,景红梅,王继军,刘彦,赵伟,宁永忠,张捷,克晓燕.血浆(1-3)-β-D-葡聚糖和半乳甘露聚糖检测在急性放射病侵袭性真菌感染防治中的临床意义[J].中华放射医学与防护杂志,2010,30(5):527-530 |
| 血浆(1-3)-β-D-葡聚糖和半乳甘露聚糖检测在急性放射病侵袭性真菌感染防治中的临床意义 |
| Diagnostic value of (1,3)-β-D-glucan assay and galactomannan test for invasive fungal infection in patients of acute radiation sickness |
| 投稿时间：2010-04-13 |
| DOI： |
| 中文关键词: 急性放射病 真菌感染 (1-3)-β-D 葡聚糖 半乳甘露聚糖 |
| 英文关键词:Acute radiation sickness(ARS) Invasive fungal infection(IFI) (1, 3)-β-D-glucan Galactomannan |
| 基金项目:卫生部公益型行业科研基金项目 (200802027) |
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| 中文摘要: |
| 目的 总结侵袭性真菌感染患者的非培养非侵入性实验室诊断方法,为事故性放射病真菌感染临床检测提供借鉴。方法 应用血浆(1-3)-β-D-葡聚糖(G)和血清半乳甘露聚糖(GM)检测方法,检测并分析了316例(次)患者的血浆或血清标本,同时,对部分患者进行常规细菌培养及真菌培养。结果 送检标本中,G及GM检测的阳性率分别为36.33%和35.84%,所有送检标本真菌培养阳性率为34.18%,而血培养真菌的阳性率仅1例,占0.3%。分析显示,G检测、GM检测、真菌培养三者和临床诊断之间具有相关性(χ2=0.564、0.357、0.727,P<0.05)。结论 (1-3)-β-D-葡聚糖(G)和血清半乳甘露聚糖(GM)两者同时检测可起到诊断的互补作用,早期发现侵袭性真菌感染,检测的阳性率高于常规真菌培养,而且检测速度明显优于常规培养的检测手段。 |
| 英文摘要: |
| Objective To investigate the diagnostic values of (1,3)-β-D-glucan (G) and galactomannan (GM) for invasive fungal infection (IFI) in patients of acute radiation sickness (ARS). Methods Samples of periogeral blood, pharyngeal secretion, urine, and feces were collected from 316 patients with ARS and suspected to suffer from IFI, 192 males and 124 females, aged 60.50(1-96), with the underlying diseases of blood or respiration systems. Platelia Aspergillus EIA kit was used to detect the plasma BG (G test), and ELISA was used to detect the serum GM (GM test). Fungal culture and bacterial culture were performed. Results The positive rates of G test, GM test, and fungal culture were 36.33%, 35.84%, and 34.18% respectively, but the positive rate of fungal culture of blood sample was 1/316 only. Pearson correlation analysis showed that G test,GM test and fungal culture test were positively correlated with IFI clinical diagnosis respectively(χ2=0.564,0.357,0.727,P<0.05). Conclusions Easy to operate, rapid, and highly sencitive, G test and GM test can be used as adjunctive methods for early IFI diagnosis in ARS patients. |
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