| 孙艳,韩云,朱应葆.人类解旋酶样转录因子过表达促进辐射诱导的A549细胞凋亡[J].中华放射医学与防护杂志,2010,30(5):507-509 |
| 人类解旋酶样转录因子过表达促进辐射诱导的A549细胞凋亡 |
| Increased apoptosis of A549 cells by overexpression of helicase-like transcription factor induced by radiation exposure |
| 投稿时间：2010-04-13 |
| DOI： |
| 中文关键词: 细胞凋亡 人类解旋酶样转录因子(HLTF) 细胞色素C |
| 英文关键词:Cell apoptosis Helicase-like transcription factor (HLTF) Cytochrome C |
| 基金项目:国家自然科学基金(30470976) |
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| 中文摘要: |
| 目的 探讨人类解旋酶样转录因子(HLTF)对辐射诱导细胞凋亡的影响及其机制。方法 将空质粒和带FLAG标签的HLTF表达质粒分别转染人肺癌细胞A549,60Co γ射线照射观察细胞凋亡变化。用Western blot 检测HLTF在细胞核和胞质内水平的变化及线粒体细胞色素C的释放。结果 HLTF转染可促进γ射线诱导的细胞凋亡,凋亡率为(32.2±2.1)%,高于空载体转染组(11.4±2.3)%和Mock转染组(11.1±1.8)%(F=101.85,P<0.01)。辐射后HLTF在胞质内的表达水平显著提高。HLTF转染组胞质内细胞色素C的水平亦显著提高。结论 HLTF可促进辐射损伤诱导的细胞凋亡, 其部分机制可能是通过提高核质转位以及影响线粒体细胞色素C的释放来实现的。 |
| 英文摘要: |
| Objective To study the effects of overexpression of helicase-like transcription factor (HLTF) on radiation-induced apoptosis of human lung carcinoma cells. Methods Human lung carcinoma cellline A549 were cultured, transfected with FLAG-tagged HLTF expression plasmid pcDNA3.1/HLTF-FLAG (HLTF gene transfection group), empty plasmid vector pcDNA3.1 (empty vector group), and mock transfection group,respectively, and then exposed to 60Co γ-ray irradiation. Cell apoptosis was observed by flow cytometry 48 h post-irradiation. Western blotting was used to detect the levels of HLTF in the nucleus and cytoplasm and the release of cytochrome C from mitochondria into cytoplasm. Results The apoptosis rate of the HLTF gene group was (32.2±2.1)%, significantly higher than those of the empty vector group ［(11.4±2.3)%］ and mock transfection group ［(11.1±1.8)%］ (F=101.85,P<0.05). The level of cytochrome C in the cytoplasm of the HLTF gene group was significantly higher than that of the empty plasmid group. Conclusions HLTF protein sensitizes human lung carcinoma cells to radiation-induced apoptosis, partly by promoting the nuclear-cytoplasma transition of HLTF protein and the increased release of cytochrome C. |
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