| 曹晓沧,杜伟廷,刘强,赵辉,杜利清,王彦,王邦茂,韩忠朝,樊飞跃.内镜冷光辐射对美蓝染色胃癌细胞SGC7901DNA损伤的研究[J].中华放射医学与防护杂志,2010,30(4):489-491 |
| 内镜冷光辐射对美蓝染色胃癌细胞SGC7901DNA损伤的研究 |
| DNA damage of gastric cancer cell line SGC7901 dyed by methylene blue after exposure to the cold light radiation of endoscope |
| 投稿时间：2009-12-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.034 |
| 中文关键词: 色素内镜 美蓝 单细胞凝胶电泳 活性氧 细胞凋亡 辐射 |
| 英文关键词:Chromoendoscopy Methylene blue Single cell gel electrophoresis ROS Cell apoptosis Radiation |
| 基金项目:国家自然科学基金(30800281);中国博士后科学基金(20080440100,200902280);教育部高等学校博士学科点专项科研基金(200800231051); 天津商业大学引进人才基金(100101Q);天津科技支撑项目基金(08ZCKFGX09400)。 |
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| 中文摘要: |
| 目的 探讨胃镜冷光源照射对美蓝(MB)染色后的胃癌细胞DNA的损伤情况,并探索损伤的发生机制和作用规律。方法 应用单细胞凝胶电泳技术,检测DNA损伤情况;Annexin V-FITC/PI双染后流式细胞仪检测细胞凋亡;采用荧光探针 DCFH-DA,进行细胞内活性氧(ROS)检测。结果 冷光照射MB染色的SGC7901细胞后,其DNA损伤程度较未照射组增加( F =8.39, P<0.05),损伤严重程度与光照时间呈正相关,光照停止则有损伤修复,照射后细胞较未照射细胞出现明显凋亡(χ2=7.71, P<0.05);同时,经照射后的细胞内ROS高于未照射组( F=34.11, P<0.01)。结论 美蓝色素内镜的冷光辐射可造成SGC7901细胞的DNA损伤,并诱发细胞凋亡,其作用机制与光化学反应激发产生的过量ROS有关。 |
| 英文摘要: |
| Objective To study the co-effects of methylene blue(MB) and exposure with the cold light source on cell DNA damage,and to explore the mechanism involved. Methods The alkaline single cell gel electrophoresis was used to determine cell DNA damage. Apoptosis of the cells was determined by flow cytometry analysis using Annexin V-FITC/PI staining. DCFH-DA probe was used to determine endocellular Reactive Oxygen Species (ROS). Results The levels of DNA damage in the SGC7901 adenocarcinoma cells treated with methylene blue in the light were significantly increased compared to that of control( F =8.39, P<0.05). The DNA damage levels were related to the length of time of light exposure, and the damage was recovered to a certain level after light withdrew. Cell apoptosis (χ2=7.71, P<0.05)and endocellular ROS level ( F =34.11, P<0.01)increased significantly in the exposure group. Conclusions Methylene blue chromoendoscopy can induce DNA damage and cell apoptosis, and the mechanism may be associated with ROS produced by the photochemical reaction. |
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