| 邢巍巍,王雪峰,金大玉.山莨菪碱对耳蜗电离辐射损伤防护作用的实验研究[J].中华放射医学与防护杂志,2010,30(4):426-429 |
| 山莨菪碱对耳蜗电离辐射损伤防护作用的实验研究 |
| Protective effect of Anisodamine on ionizing radiation damage of cochlea |
| 投稿时间：2009-11-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.013 |
| 中文关键词: 辐射损伤 耳蜗 豚鼠 山莨菪碱 放射保护 |
| 英文关键词:Radiational damage Cochlea Guinea pigs Anisodamine Radioprotective effect |
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| 中文摘要: |
| 目的 观察电离辐射对耳蜗毛细胞的损伤情况,探讨山莨菪碱对耳蜗辐射损伤的防护作用。方法 将健康豚鼠50只随机分成3组,健康对照组10只,单纯照射组和药物防护组各20只,每组观察20耳。各组豚鼠行听觉脑干反应(ABR)阈值测定后,对药物防护组和单纯照射组豚鼠的耳颞部用直线加速器所产生的6 MeV电子线予以分次放射(2 Gy/d),每次照射前30 min于药物防护组豚鼠的股部肌肉注射山莨菪碱,单纯照射组在同一部位注射等量生理盐水,总剂量达到60 Gy后,各组豚鼠行ABR检测听功能。并通过光镜、透射电镜及扫描电镜观察耳蜗毛细胞的形态学变化。结果 放射后单纯照射组的平均ABR阈值为(52.27±2.42)dB peSPL,较药物防护组(37.65±1.92)dB peSPL明显提高 ( t =2.01, P<0.05);耳蜗基底膜铺片外毛细胞计数,单纯照射组为(54.75±7.71)个/视野,较药物防护组(144.50±7.30)个/视野明显减少( F =135.362, P<0.05)。透射电镜观察见单纯照射耳蜗外毛细胞边界不清,细胞肿胀变形,细胞器和细胞核明显异常;防护组耳蜗外毛细胞病变明显减轻;健康对照组外毛细胞完好。扫描电镜观察见单纯照射组耳蜗外毛细胞的纤毛明显倒伏、缺失、排列紊乱;药物防护组耳蜗外毛细胞的纤毛排列基本整齐,偶见倒伏现象;健康对照组耳蜗外毛细胞的纤毛排列整齐无倒伏、缺失。 结论 分割剂量60 Gy对豚鼠耳颞部放射可造成耳蜗毛细胞损害,山莨菪碱对耳蜗辐射损伤具有保护作用。 |
| 英文摘要: |
| Objective To explore protective effect of Anisodamine against injure of cochlea hair cells induced by ionizing radiation.Methods 50 healthy guinea pigs were randomly divided into 3 groups: radiation group(n=20), anisodamine protective group(n=20) and control group(n=10). 20 ears were observed in each group. Tempora of guinea pigs in anisodamine protective group and radiation group were exposed to fractional doses of 6 MeV electron beam produced by linear accelerators (2 Gy/d) with a total dose of 60 Gy. Animals were injected intramuscularly with anisodamine (20 mg/kg) in protection group and equal saline solution in irradiated group, respectively, 30 min pre-irradiation. Morphological and functional changes in the guinea pig cochlea were observed.Results After radiation, the average ABR threshold value of the radiation group (52.27±2.42 dB peSPL) was significantly higher than that of the anisodamine protection group (37.65±1.92 dB peSPL, t =2.01, P<0.05), and outer fair cells of cochlea reduced greatly in quantity by observing the stretched preparations of cochlea basilar membrane( F= 135.362, P<0.05).Under transmission electron microscope, outer fair cells of cochlea in the radiation group had obscure boundaries and swelled to lose their original shapes. Meanwhile, cell implement and the nucleus were obviously obnormal after radiation. In the anisodamine protection group, the boundaries of cochlea outer fair cells were very clear and the cells swelled a little. Cell implement and nuclear envelope were complete. Cochlea outer fair cells in the control group were found to line up in order with no collapse and reduction in number under scanning electron microscope, but those in the radiation group collapsed obviously, reduced in number and aligned in a state of chaos, while the outer fair cells in the anisodamine protection group regularly posed on the whole and the collapse was occasionally found.Conclusions The 60 Gy radiation at fractioned dose on the tempuses of the guinea pigs could cause damage to the cochlea hair cells. Anisodamine might have a protective effect against radiation damage. |
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