中华放射医学与防护杂志

孙维凯,李文,杨香桃,方建萍,朱伟宏.分次局部照射对H22肝癌移植小鼠肺转移的影响及其机制探讨[J].中华放射医学与防护杂志,2010,30(4):423-425

分次局部照射对H22肝癌移植小鼠肺转移的影响及其机制探讨

Impact of fractionated local irradiation on lung metastasis in H22-bearing mice and exploration of its mechanism

投稿时间：2009-05-04

DOI：10.3760/cma.j.issn.0254-5098.2010.04.012

英文关键词:Neoplasm transplantation Mice Irradiation Neoplasm metastasis Lung

基金项目:

作者	单位
孙维凯	200065 上海, 同济大学附属同济医院放疗科
李文	200065 上海, 同济大学附属同济医院放疗科
杨香桃	200065 上海, 同济大学附属同济医院放疗科
方建萍	200065 上海, 同济大学附属同济医院病理科(方建萍)
朱伟宏	同济大学附属第十人民医院胸外科

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中文摘要:

目的研究分次局部照射对H22肝癌移植小鼠肺转移的影响,并探讨其机制。方法建立昆明小鼠皮下H22肝癌移植模型,总计96只小鼠随机分为健康对照组、肿瘤对照组和肿瘤照射组。观察局部肿瘤生长和肺转移情况,并进一步研究肿瘤增殖核抗原计数(PCNA)、血管内皮生长因子(VEGF)、肿瘤内微血管密度(MVD)及血浆T细胞亚群变化。结果肿瘤照射组皮下肿瘤缩小,抑瘤率约为30%,但该组大体和镜下肺转移数及死亡数较肿瘤对照组高(χ²=8.31、4.48、9.60, P<0.05),转移结节大多明显。肿瘤对照组和照射组PCNA、 VEGF、 MVD表达差异均有统计学意义( t =23.78、-2.47、-6.43, P<0.05)。肿瘤照射组血浆T细胞亚群CD4和CD8较健康组有明显下降( t =4.72和3.31, P<0.05)。结论局部照射H22肝癌移植小鼠模型可使皮下肿瘤缩小,但有可能增加肺转移风险。

英文摘要:

Objective To study the impact of local fractionated irradiation on lung metastasis in H22-bearing mice, and to explore its mechanism involved. Methods Subcutaneous transplantation tumor model bearing with H22 was established. Mice were divided into three groups as healthy control, tumor control and irradiation groups. The size of subcutaneous tumors was measured and lung metastasis was observed. The expressions of PCNA,VEGF and MVD were detected immunohistochemically.The plasma levels of CD4 and CD8 were determined by using flow cytometry. Results The tumor size in irradiated group was smaller than that in tumor control group. The tumor inhibition rate in irradiated group was 30%. Lung metastasis in irradiated group was more severe than that in tumor control group( χ²=8.31,4.48,9.60, P<0.05 ) . The expressions of PCNA,VEGF and MVD in two groups were statistically different( t =23.78,-2.47,-6.43, P<0.05). The levels of CD4 and CD8 in irradiated group were statistically different compared to healthy control group( t =4.72 and 3.31, P<0.05). Conclusions For the H22 model, radiation might inhibit the local transplantation tumor, but increase the risk of lung metastasis.

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