| 杨青山,刘媛媛,樊飞跃.Ku80基因沉默联合γ射线对人食管癌细胞移植瘤生长的影响[J].中华放射医学与防护杂志,2010,30(4):407-409.YANG Qing-shan,LIU Yuan-yuan,FAN Fei-yue.Effect of silencing Ku80 combined with γ-rays on growth of esophageal cancer xenografts[J].Chin J Radiol Med Prot,2010,30(4):407-409 |
| Ku80基因沉默联合γ射线对人食管癌细胞移植瘤生长的影响 |
| Effect of silencing Ku80 combined with γ-rays on growth of esophageal cancer xenografts |
| 投稿时间：2010-01-06 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.008 |
| 中文关键词: shRNA Ku80 移植瘤 放射 |
| 英文关键词:shRNA Ku80 Esophageal cancer xenografs Radiation |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨shRNA抑制Ku80蛋白表达联合照射对裸鼠移植瘤食管癌生长的抑制作用。 方法 构建干扰Ku80载体shRNA-Ku80,采用Western blotting方法观察shRNA干扰Ku80蛋白的表达。将20只裸鼠随机分为对照组、单纯照射组、shRNA-H2组和联合治疗组,观察裸鼠移植瘤的生长情况。免疫组织化学法检测移植瘤内Ku80表达。结果 成功构建了shRNA-Ku80载体,并挑选出有效的靶序列。Ku80干扰载体和放射对移植瘤生长均有抑制作用,抑瘤率分别为32.0%和39.9%。二者联合作用抑制更显著,抑瘤率为68.9%。shRNA-H2降低移植瘤内Ku80表达58%(t=3.77,P<0.05)。结论 shRNA干扰Ku80联合照射能显著提高食管癌细胞移植瘤的放射效应。 |
| 英文摘要: |
| Objective To investigate the inhibition effect of silencing Ku80 gene combined with irradiation on growth of esophageal cancer xenografs.Methods shRNA-Ku80 vector was constructed. The expression of Ku80 protein was inhibited by shRNA-Ku80 vector by using Western blotting. 20 BALB/c nude mice were randomly divided into 4 groups, including control group, radiation group, shRNA-Ku80 group and combined group. The growth of esophageal cancer xenografs was observed. The expression of Ku80 was examined in esophageal cancer xenografs by IHC.Results Effective target sequence was selected. The growth of esophageal cancer xenografs was inhibited by shRNA-H2 and radiation, especially in combined group. The inhibition rate of growth in three groups above was 32.0%, 39.9% and 68.9%, respectively. The expression of Ku80 was reduced to 58% by shRNA-H2 in esophageal cancer xenografs (t=3.77, P<0.05).Conclusions Combination of silencing Ku80 and radiation could enhance radiotherapeut effect of esophageal cancer xenografs. |
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