| 封巍,郑晓,王跃珍,王准,祝淑钗.干扰血管内皮生长因子表达联合γ射线对食管癌细胞移植瘤的影响[J].中华放射医学与防护杂志,2010,30(4):395-398 |
| 干扰血管内皮生长因子表达联合γ射线对食管癌细胞移植瘤的影响 |
| Effect of γ-rays combined with blocking VEGF expression on esophageal cancer xenografts |
| 投稿时间：2009-07-20 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.005 |
| 中文关键词: 食管癌细胞TE-1 血管内皮生长因子 移植瘤 放射 |
| 英文关键词:Esophageal cancer cell TE-1 Vascular endothelial growth factor (VEGF) Xenografts Irradiation |
| 基金项目:浙江省医药卫生优秀青年科技人才专项基金(2008QN005);浙江省省医药卫生科学研究基金项目(C2006B011);卫生部科学研究基金-浙江省医药卫生重大科技计划项目 (WKJ2007-2-004) |
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| 中文摘要: |
| 目的 研究反义cDNA干扰血管内皮生长因子(VEGF)表达联合γ射线对裸鼠移植瘤的影响,并观察各组裸鼠肿瘤病理组织学和细胞生物学特征的变化,为VEGF基因治疗联合放射治疗食管癌提供理论依据。方法 将32只Balb/c/nu裸鼠随机分为4组:对照组、单纯照射组、反义组和反义+照射组,使用已转染和未转染反义VEGFcDNA TE-1食管癌细胞株,分别建立裸鼠爪垫移植瘤模型, 瘤体直径0.8~1.0 cm,60Co γ射线18 Gy单次照射单纯照射组与反义+照射组裸鼠移植瘤,对比观察各组移植瘤生长情况,检测移植瘤组织中VEGF mRNA和蛋白的表达,并分析肿瘤组织中细胞凋亡情况。结果 与未转染两组比较,转染两组瘤体生长速度慢,成瘤潜伏期延长(t=13.898, P<0.01)。反义组肿瘤体积为(1207.50±97.07)mm3,反义+照射组为(1057.5±91.50)mm3,两者差异无统计学意义(t=1.124,P>0.05),而此2组与对照组(5442.50±185.08)mm3 和单纯照射组(2922.50±152.773)mm3 体积间差异有统计学意义(t=9.475~21.238,P<0.01)。反义组与反义+照射组VEGF mRNA和蛋白表达较对照组和单纯照射组差异有统计学意义(F=387.394、13.519, P<0.01)。结论 抗VEGF治疗可有效地抑制裸鼠移植瘤的生长,但单纯抑制VEGF表达对增加裸鼠移植瘤放射治疗增敏效果有限,需进一步研究。 |
| 英文摘要: |
| Objective To investigate the effect of blocking VEGF expression on the radiation sensitivity of esophageal cancer cell line TE-1 in vivo.Methods 32 male Balb/c/nu nude mice were randomly divided into four groups, including control group, radiation group, anti-VEGF group, and anti-VEGF + radiation group. The anti-VEGFcDNA cells were subcutaneously injected into the paw pats of mice(2×106/100 μl).The subcutaneous tumors were irradiated with 18 Gy of 60Co γ-rays when the diameter of tumors varied from 0.8 to 1.0 cm. The volume of the tumors was measured before and after irradiation, respectively. The expression level of VEGF mRNA and protein were examined by RT-PCR and Western blotting, respectively. Apoptotic cells were detected by electron micrographs. Results Latent period of the tumor formation of anti-VEGF group was lengthened compared with other groups(t=13.898, P<0.01).The volumes of tumor in anti-VEGF group[(1207.50±97.07)mm3] and anti-VEGF + radiation group [(1057.5±91.50)mm3] were not statistically different post-irradiation (t=1.124,P>0.05), but smaller than those in control group [(5442.50±185.08)mm3] and radiation group [(2922.50±152.773)mm3] with statistical differences(t=9.475-21.238,P<0.01). The expression level of endogenous VEGFmRNA and protein in anti-VEGF group and anti-VEGF + radiation group were statistically different from control group and radiation group (F=387.394, 13.519, P<0.01).Conclusions Antisense VEGF could inhibit the proliferation of esophageal cancer cell in the nude mice. Effect of blocking VEGF expression before irradiation on esophageal cancer xenografts might be limited. |
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