| 朱小东,苏芳,曲颂,王琪,李力,张玮.鼻咽癌放疗前后血清蛋白质谱的变异性研究[J].中华放射医学与防护杂志,2010,30(4):391-394 |
| 鼻咽癌放疗前后血清蛋白质谱的变异性研究 |
| Variabilities of serum proteomic spectra in patients with nasopharyngeal carcinoma before and after radiotherapy |
| 投稿时间：2009-10-29 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.004 |
| 中文关键词: 鼻咽癌 表面增强激光解吸离子化飞行时间质谱技术 蛋白质标志物 |
| 英文关键词:Nasopharyngeal carcinoma Surface-enhanced laser desorption ionization time-of flight-mass spectrometry Protein biomarker |
| 基金项目:吴阶平医学基金会临床科研专项资助基金(320.6750.06080);广西科学研究与技术开发计划课题(桂科攻0719006-2-23);广西研究生教育创新计划资助项目(2007105981002 M33);广西大型仪器协作共用网测试补助项目(533-2007-112;555-2007-134) |
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| 中文摘要: |
| 目的 通过对鼻咽癌患者放疗前后及健康对照组血清蛋白质谱的测定,观察放疗前后血清蛋白质图谱变化。方法 应用CM-10弱阳离子交换芯片和表面增强激光解吸离子化飞行时间质谱技术(SELDI-TOF-MS),分析50例鼻咽癌放疗前组、40例健康对照组和25例经放疗后完全缓解组(鼻咽癌患者自身配对)3组的血清标本。结果 对鼻咽癌放疗前组与正常对照组蛋白质谱分析发现共有20个蛋白质表达量有差异。获得相对分子质量为2931、4098、5343和13766的4个蛋白质峰组合建立的分类树模型,判别鼻咽癌的敏感性为90.0%,特异性为90.0%。观察20个差异蛋白质的表达量在放疗后的变化,显示均发生了不同程度的变化。放疗前组高表达的相对分子质量为2931、4182、4688、13766的4个蛋白质表达量在完全缓解组降低,接近正常水平;相对分子质量为4098和5343的蛋白质表达量在CR组升高,接近正常水平。结论 应用SELDI-TOF-MS分析,可在放疗前后筛选出鼻咽癌患者血清蛋白质标志物,有可能对微小病灶的监测及疗效的判定等提供有用的信息。 |
| 英文摘要: |
| Objective To study the changes of serum proteomic spectra in patients with nasopharyngeal carcinoma(NPC) before and after treatment in order to detect the protein biomarkers. Methods Proteomic spectra from serum of 50 NPC patients before radiotherapy,25 NPC patients who achieved complete remission(CR) after radiotherapy, and 40 persons from normal control subjects were analyzed by CM-10 protein chip and surface-enhanced laser desorption ionization time of flight mass spectrometry. Results Expressed proteins in serum were screened by analysis of the proteomic spectra of pre-radiotherapy patients and normal individuals. 4 kinds of proteins with the relative molecular masses of 2931,4098,5343,13766 made up markers pattern which was able to classify the patients and normal individuals. The sensitivity and specificity results were 90.0% and 90.0%, respectively. The twenty differential expression protein peaks of patients before and after radiotherapy were obviously different. The relative molecular masses of 2931, 4182, 4688 and 13766 were up-regulated in untreated NPC, while were close to the normal levels in CR group. Two other protein peaks of 4098 and 5343 were down-regulated in untreated NPC group, which were close to normal levels in CR group.Conclusions The expressions of protein levels are different before and after radiotherapy in NPC patients. Protein signatures of NPC may be screened using SELDI-TOF-MS. Those signatures may be helpful in assessing the minimal residual disease and predicting the treatment efficacy. |
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