中华放射医学与防护杂志

李海英,张力,吴建波,等.西妥昔单抗对人食管癌放射抗拒性细胞株KYSE-150R放射敏感性的研究[J].中华放射医学与防护杂志,2010,30(3):291-294.LI Hai-ying,ZHANG Li,WU Jian-bo,et al.Cetuximab enhances the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R[J].Chin J Radiol Med Prot,2010,30(3):291-294

西妥昔单抗对人食管癌放射抗拒性细胞株KYSE-150R放射敏感性的研究

Cetuximab enhances the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R

投稿时间：2009-07-13

DOI：10.3760/cma.j.issn.0254-5098.2010.03.016

中文关键词: 食管癌放射敏感性西妥昔单抗

英文关键词:Esophageal carcinoma Radiosensitivity Cetuximab

基金项目:

作者	单位	E-mail
李海英	325000, 温州医学院附属第一医院医科所
张力	温州医学院附属第一医院放疗科
吴建波	325000, 温州医学院附属第一医院医科所
苏华芳	温州医学院附属第一医院放疗科
吴式琇	温州医学院附属第一医院放疗科	wushixiu@medmail.com.cn

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中文摘要:

目的观察西妥昔单抗(C225)对人食管鳞癌放射抗拒性细胞株KYSE-150R的影响。方法分次照射建立人食管鳞癌放射抗拒性细胞株KYSE-150R;相差显微镜下观察细胞的形态学差异;G显带法进行染色体分析;克隆形成实验验证KYSE-150和KYSE-150R细胞的不同放射敏感性;采用不同浓度的C225处理KYSE-150R细胞,研究对KYSE-150R细胞放射敏感性的影响。结果 KYSE-150R细胞的群体倍增时间为(25.9±0.6)h,长于亲代细胞的KYSE-150(23.6±0.2) h(t=6.6,P＜0.01);KYSE-150R细胞的染色体数目由69.3±1.9 增加到73.7±1.2(t=-8.83,P<0.01),并且观察到有染色体畸变;KYSE-150R细胞SF₂、D₀、D_q及N 值均高于KYSE-150细胞, 加入5 μg/ml的C225后,其SF₂、D₀、D_q、N与单纯照射组KYSE-150R细胞相比有显著降低;加入C225后KYSE-150R细胞G₀/G₁、G₂/M期比例增加,S期比例减少(t=-4.478~4.308, P<0.05)。结论 C225对人食管鳞癌放射抗拒性细胞株KYSE-150R有放射增敏作用。

英文摘要:

Objective To determine the effect of cetuximab (C225) on the radioresistant human esophageal squamous carcinoma cell line KYSE-150R.Methods A radioresistant human esophageal squamous carcinoma cell line KYSE-150R was established by fractionated irradiation. Morphological changes from KYSE-150 to KYSE-150R were observed by phase-contrast microscopy. Karyotype analysis was performed by G-banding. The radiosensitivities were analyzed by colony formation assays.Results The population doubling time of KYSE-150 and KYSE-150R were (23.6±0.2) h and (25.9±0.6) h(t=6.6,P＜0.01), respectively. The chromosome number of KYSE-150R was increased and chromosome aberrations were observed from (69.3±1.9) h to (73.7±1.2)h (t=-8.83,P<0.01). The SF₂, D₀, D_q and N values of KYSE-150R were all higher than those of KYSE-150. After 5 μg/ml of C225 added, the SF₂, D₀, D_q and N values were significantly decreased as compared to the control. After C225 treatment, the G₀/G₁ and G₂/M phase cells were increased,while S-phase cells decreased(t=-4.478-4.308, P<0.05).Conclusion Cetuximab can enhance the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R.

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