| 于静萍,孙苏平,孙志强,等.沙利度胺对食管癌TE1细胞DNA合成抑制作用和辐射敏感性的影响[J].中华放射医学与防护杂志,2010,30(3):270-273.YU Jing-ping,SUN Su-ping,SUN Zhi-qiang,et al.Inhibition of DNA synthesis and radiosensization effects of thalidomide on esophageal carcinoma TE1 cells[J].Chin J Radiol Med Prot,2010,30(3):270-273 |
| 沙利度胺对食管癌TE1细胞DNA合成抑制作用和辐射敏感性的影响 |
| Inhibition of DNA synthesis and radiosensization effects of thalidomide on esophageal carcinoma TE1 cells |
| 投稿时间：2009-11-03 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.010 |
| 中文关键词: 沙利度胺 TE1细胞 DNA合成 辐射敏感性 |
| 英文关键词:Thalidomide TE1 cell DNA synthesis Radiosensitivity |
| 基金项目:国家自然科学基金(30870585);常州市青年科技培养计划(CQ2008006);常州市社会发展指导性项目(CS2008920) |
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| 中文摘要: |
| 目的 探讨不同浓度沙利度胺联合X线照射对食管癌TE1细胞放射敏感性的影响。方法 采用细胞划痕实验检测沙利度胺对细胞浸润转移能力的影响,H3-TdR掺入法研究沙利度胺单用及联合X射线对TE1细胞DNA合成抑制作用,用克隆形成法研究对TE1细胞辐射敏感性的影响。结果 沙利度胺对食管癌TE1细胞的浸润转移能力、DNA合成、克隆形成均有明显的抑制作用,并随药物浓度的升高而增强。TE1细胞的D0、Dq、SF2值随着沙利度胺浓度的增加而减小:沙利度胺100 μg/ml时放射增敏比SERD0和SERDq分别为1.4±0.2和1.5±0.1;150 μg/ml时的放射增敏比分别为1.4±0.2和1.8±0.2。结论 沙利度胺能够抑制TE1细胞的浸润转移、DNA合成能力,显著提高TE1细胞的辐射敏感性。 |
| 英文摘要: |
| Objective To explore the radiosensitization effect of thalidomide combined with X-ray on esophageal carcinoma TE1 cells. Methods Cell scratch assay was used to detect the inhibition ability of different concentration of Thalidomide on cell invasion and metastasis. H3-TdR incorporation assay was used to investigate the inhibition of DNA synthesis in TE1 cells by treated with Thalidomide singly or combination with X-rays. The colony formation assay was used to analyze the radiosensitization of Thalidomide effect on TE1 cells. Results Thalidomide had obvious inhibition effect on TE1 cell metastasis, DNA synthesis and colony formation, which were correlated with drug concentration. The values D0, Dq and SF2 in TE1 cells were gradually decreased with thalidomide concentration increased. When the concentration of thalidomide was 100μg/ml, the SERD0 and SERDq were (1.4±0.2) and (1.5±0.1), respectively, While the concentration of thalidomide was 150 μg/ml, the SERD0 and SERDq were (1.5±0.2) and (1.8±0.2), respectively. Conclusions Thalidomide could inhibit TE1 cell invasion, metastasis, DNA synthesis, and significantly enhance the radiosensitizing effect on esophageal carcinoma TE1 cells. |
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