| 贾立立,张涛,杨永华,等.单细胞凝胶电泳用于放射工作者DNA损伤评价的研究[J].中华放射医学与防护杂志,2010,30(3):267-269.JIA Li-li,ZHANG Tao,YANG Yong-hua,et al.Assessment of DNA damage in radiation workers by using single cell gel electrophoresis[J].Chin J Radiol Med Prot,2010,30(3):267-269 |
| 单细胞凝胶电泳用于放射工作者DNA损伤评价的研究 |
| Assessment of DNA damage in radiation workers by using single cell gel electrophoresis |
| 投稿时间：2009-12-16 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.009 |
| 中文关键词: 电离辐射 放射性工作者 单细胞凝胶电泳 DNA损伤 彗星实验 |
| 英文关键词:Ionizing radiation Radiation workers DNA damage Single cell gel electrophoresis |
| 基金项目:国家自然科学基金项目(30800281);天津市自然科学基金(07JCYBJC09200) |
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| 中文摘要: |
| 目的 评价不同级别医院放射工作人员的DNA损伤情况,探讨不同工种、工龄与DNA损伤程度之间的关系。方法 用碱性单细胞凝胶电泳技术检测放射组和对照组外周血淋巴细胞的DNA单链断裂水平。CASP软件分析彗星图像,主要观察彗星尾部DNA百分含量(TDNA%)、彗星尾长(TL)、尾矩(TM)和Olive尾矩(OTM)等指标。 结果 放射组的TDNA%,TL、TM、OTM明显高于对照组(F=3.93, P<0.01),不同工种、工龄间均有统计学差异(F=1.83, P<0.05),不同级别医院之间放射工作者上述指标也差异存在统计学意义(F=1.91,P<0.05)。结论 所观察医院的放射工作者外周血淋巴细胞DNA均存在不同程度的损伤,高级别医院略好于低级别医院,而且DNA损伤程度与放射工龄和工种有一定的相关性。 |
| 英文摘要: |
| Objective To assess the DNA damage of radiation workers in different grade hospitals, and to explore the correlation between the types of work or work time and the levels of DNA damage. Methods DNA single strand break were detected by using alkaline single cell gel electrophoresis(SCGE), and the comet was analyzed with CASP(Comet Assay Software Project). TDNA%, TL, TM and OTM were calculated. Results The parameters of SCGE in the radiation group were higher than those of control group (F=3.93,P<0.01). The significant difference was found not only among the different types of work or different work time, but also among the different grade hospitals(F=1.83,1.91,P <0.05). Conclusions Various levels of DNA damage could be detected in the radiation workers of the two hospitals. DNA damage of radiation workers is less serious in the higher-grade hospital than the lower grade one.Different types of work or work time might affect the DNA damage level. |
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