| 王治东,葛常辉,许望翔,詹轶群,汤立宣,曹萌萌,杨晓明.CBLB502蛋白原核表达及辐射防护作用验证[J].中华放射医学与防护杂志,2010,30(2):169-172 |
| CBLB502蛋白原核表达及辐射防护作用验证 |
| Expression of CBLB502 in prokaryocytes and verification of radiation protective effect |
| 投稿时间：2009-08-21 |
| DOI： |
| 中文关键词: TLR5 CBLB502蛋白 辐射防护 |
| 英文关键词:Toll-like receptor 5 CBLB502 protein Radiation protection |
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| 全文下载次数: 2022 |
| 中文摘要: |
| 目的 原核表达并纯化TLR5受体的激动剂CBLB502蛋白,验证其辐射防护作用。方法 采用pET28b原核表达系统表达CBLB502蛋白,利用Ni-NTA亲和层析柱亲和层析纯化CBLB502蛋白,经报告基因检测方法结合动物实验评价纯化蛋白活性。结果 成功表达并纯化CBLB502蛋白,纯度可达95%以上,能够激活NF-κB报告基因,可以提高9 Gy照射小鼠存活率,对照组小鼠全部死亡,CBLB502蛋白预防组全部存活。结论 成功表达并纯化CBLB502蛋白,并验证其对小鼠的辐射防护作用。 |
| 英文摘要: |
| Objective To obtain the purified CBLB502 protein as an agonist of Toll like receptor 5 in prokaryocytes and to validate its radiation protective effect. Methods pET28b expression system was used to express CBLB502 protein, and the Ni-NTA agarose and purification column was used to purify CBLB502 protein. The radiation proective effect of CBLB502 was evaluated by NF-κB reporter gene assay and mice experiments. Results CBLB502 protein was expressed and purified, which could activate NF-κB reporter gene in vitro and improved the livability of irradiated mice by 9 Gy γ-ray.Conclusions CBLB502 protein could be successfully expressed and purified using prokaryotic expression system. It has the excellent radiation protective effect in mice. |
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