| 武宁,刘丽波,张萱,金顺子,倪冠英,刘宇光,刘树铮.X射线全身照射诱导小鼠胸腺Egr-1和Egr-4基因表达水平的实时定量RT-PCR分析[J].中华放射医学与防护杂志,2010,30(2):147-151 |
| X射线全身照射诱导小鼠胸腺Egr-1和Egr-4基因表达水平的实时定量RT-PCR分析 |
| Quantitative detection of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation with X-rays by real-time RT-PCR |
| 投稿时间：2009-03-10 |
| DOI： |
| 中文关键词: 实时定量RT-PCR Egr-1 Egr-4 骨髓嗜多染红细胞微核 辐射生物剂量计 |
| 英文关键词:Real-time quantitative RT-PCR Egr-1 Egr-4 Bone marrow polychromatic erythrocyte micronucleus Radiation biodosimeter |
| 基金项目:卫生部卫生行业科研专项基金(200802018) |
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| 中文摘要: |
| 目的 应用实时定量RT-PCR技术检测X射线全身照射诱导小鼠胸腺Egr-1和Egr-4基因的表达变化。方法 小鼠接受X射线全身照射后4和24 h,提取胸腺mRNA,以GAPDH作为内参基因,利用实时定量RT-PCR检测方法,探讨Egr-1和Egr-4基因的相对表达量与不同照射剂量之间的关系。同时计数骨髓嗜多染红细胞微核率作为辐射生物剂量参照。结果 Egr-1和Egr-4基因表达量在0.5～6 Gy全身照射后4和24 h均与照射剂量之间存在显著的相关性(r =0.974,0.987,0.978,0.999, P＜0.01),其剂量效应曲线可拟合成线性平方方程。各剂量点Egr-1和Egr-4基因表达量与骨髓嗜多染红细胞微核率高度相关(r =0.866、0.947、0.983、0.835, P＜0.05)。进一步分析表明,照射后4 h Egr-4基因表达不仅剂量效应关系良好,且增幅最为明显,并与骨髓嗜多染红细胞微核率的剂量效应曲线相平行。结论 实时定量RT-PCR技术检测Egr-4基因的早期表达有可能成为建立大批量、快速辐射生物剂量估算方法的候选者。 |
| 英文摘要: |
| Objective To investigate the changes of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation(WBI)with X-rays by real-time quantitative reverse transcription-polymerase chain reaction(QRT-PCR). Methods The mRNA was isolated from mice thymus 4 and 24 h after WBI with 0-6 Gy of X-ray irradiation. The changes of Egr-1 and Egr-4 gene expression 4 and 24 h post irradiation were examined with QRT-PCR. Parallelled counts of micronucleus rate in bone marrow polychromatic erythrocytes(PCE)was made as a reference radiation biodosimetric control.Results The relative expression of Egr-1 and Egr-4 genes at 4 and 24 h after WBI with 0.5 to 6 Gy was changed with the dose (r =0.974,0.987,0.978,0.999, P＜0.01).At all dose points the relative expression of Egr-1 and Egr-4 genes was highly correlated with the micronucleus rate of bone marrow PCE(r =0.866,0.947,0.983,0.835, P＜0.05). The dose-effect relationship could be fitted into linear-quadratic model. The expression of Egr-4 gene was significantly increased at 4 h post irradiation and best correlated with PCE micronucleus rate. Conclusions QRT-PCR assay of early expression of Egr-4 gene might be a candidate for fast, high-throughput radiation biodosimetry. |
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