| 高琳,刘晓冬,马淑梅.beclin1在辐射诱导的乳腺癌细胞自噬发生中表达的变化[J].中华放射医学与防护杂志,2010,30(2):124-128 |
| beclin1在辐射诱导的乳腺癌细胞自噬发生中表达的变化 |
| Changes of beclin 1 expression in radiation-induced autophagy in breast cancer cells |
| 投稿时间：2009-11-03 |
| DOI： |
| 中文关键词: 自噬 X射线 MAP1LC3B beclin 1 |
| 英文关键词:Autophagy X-rays MAP1LC3B beclin 1 |
| 基金项目:国家自然科学基金(30770649,30970682) |
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| 摘要点击次数: 2564 |
| 全文下载次数: 2075 |
| 中文摘要: |
| 目的 研究不同剂量X射线诱导乳腺癌细胞株MCF-7自噬发生过程中beclin 1表达的变化和作用。 方法 采用实时荧光定量PCR方法检测自噬相关基因MAP1LC3B表达,RT-PCR方法检测beclin 1基因变化,X射线Western blot方法检测beclin 1蛋白表达。结果 MAP1LC3B mRNA量效研究表明,8、16和32 h量效研究均发现表达上调,8、16 h在12 Gy,32 h在8 Gy达到表达峰值,总体表现为表达升高(F =13.831、10.996、17.019, P＜0.05);时程研究表明,2 Gy时程研究发现MAP1LC3B于16 h升高达至峰值(F =16.284, P＜0.01),8 Gy时程研究中32 h升高达至峰值(F =9.030, P＜0.05),12 Gy时程研究中16 h升高达至峰值(F =20.315, P＜0.05)。beclin 1 mRNA量效关系表明,照射后4 h beclin 1在2、4和8 Gy阶段呈剂量依赖增加趋势;8 Gy时间效应关系中发现照射后beclin 1 mRNA的变化呈时间依赖性增加。16 h蛋白量效关系表明,照射后beclin 1表达增加;2 Gy时效关系表明照射后蛋白表达增加。 结论 X射线照射能够诱导乳腺癌细胞株MCF-7发生自噬,beclin 1基因上调表达的变化提示其发挥重要作用。 |
| 英文摘要: |
| Objective To investigate the changes of beclin 1 expression in radiation-induced autophagy in breast cancer cell line MCF-7.Methods Real-time RT-PCR was used to detect the expression of autophagy-related genes MAP1LC3B.Reverse transcription-PCR was used to detect the expression of beclin 1.Western blot was used to analyze the protein expression of beclin 1.Results Dose-response of MAP1LC3B mRNA studies showed that MAP1LC3B mRNA increased in 8, 16 and 32 h dose-effect studies, up to peak at 12 Gy in 8 h study(F =13.831, P＜0.05), 12 Gy in 16 h study (F =10.996, P＜0.01) and 8 Gy in 32 h study (F =17.019, P＜0.01), respectively. Time-response studies showed that MAP1LC3B mRNA increased and reached to peak at 16 h in 2 Gy dose-effect study (F =16.284, P＜0.01), 32 h in 8 Gy study (F =9.030, P＜0.05) and 16 h in 12 Gy study (F =20.315, P＜0.05), respectively.Dose-response studies of beclin 1 mRNA showed that beclin 1 increased after 2, 4 and 8 Gy irradiation. Time-response studies showed that beclin 1 increased in a time-dependent manner after 8 Gy irradiation.16 h dose-effect study of beclin 1 protein showed the increase of beclin 1 expression, and 2 Gy time-response study showed that beclin 1 expression increased and reached the peak at 8 h.Conclusions X-ray irradiation could induce autophagy in MCF-7 cells. beclin 1 might play an important role in the process of autophagy. |
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