| 刘汉臣,李雨,李百龙,崔建国,高福,孙顶,单鹄声,赵芳,黄越承,董俊瑞,刘浩,蔡建明.羧酸富勒烯C3对K562和AHH-1细胞的辐射生物效应研究[J].中华放射医学与防护杂志,2010,30(1):1-4 |
| 羧酸富勒烯C3对K562和AHH-1细胞的辐射生物效应研究 |
| Differential radiobiological effects of carboxyfullerene C3on K562 and AHH-1 cells |
| 投稿时间：2009-06-26 |
| DOI： |
| 中文关键词: 羧酸富勒烯C3 K562细胞 AHH-1细胞 γ射线 细胞凋亡 |
| 英文关键词:Carboxyfullerene C3 K562 cell AHH-1 cell γ-ray Apoptosis |
| 基金项目:国家自然科学基金(30570552);全军医药卫生"十一五"攻关课题(06G60) |
| 作者 | 单位 | E-mail | | 刘汉臣 | 200433 上海, 第二军医大学放射医学研究室 | | | 李雨 | 200433 上海, 第二军医大学放射医学研究室 | | | 李百龙 | 200433 上海, 第二军医大学放射医学研究室 | | | 崔建国 | 200433 上海, 第二军医大学放射医学研究室 | | | 高福 | 200433 上海, 第二军医大学放射医学研究室 | | | 孙顶 | 200433 上海, 第二军医大学放射医学研究室 | | | 单鹄声 | 200433 上海, 第二军医大学放射医学研究室 | | | 赵芳 | 200433 上海, 第二军医大学放射医学研究室 | | | 黄越承 | 200433 上海, 第二军医大学放射医学研究室 | | | 董俊瑞 | 200433 上海, 第二军医大学放射医学研究室 | | | 刘浩 | 200433 上海, 第二军医大学放射医学研究室 | | | 蔡建明 | 200433 上海, 第二军医大学放射医学研究室 | cjm882003@yahoo.com.cn |
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| 中文摘要: |
| 目的 探讨羧酸富勒烯C3(C3)在辐射防护和辅助放疗方面的应用。方法 以不同浓度C3与AHH-1、K562细胞共孵育,用CCK-8法、锥虫蓝试验检测C3对细胞活力和存活率的影响,用Annexin-V/PI染色、流式细胞分析法研究照射后细胞凋亡和细胞周期的变化。结果 C3对AHH-1细胞活力几乎无影响(存活率大于95%),但600mg/L的C3使K562细胞存活率明显降低(82%)。100mg/LC3用药照射组与单纯照射组相比,4Gy照射后AHH-1细胞的存活率显著升高(71.3%、90.3%),细胞凋亡率明显下降(26.3%、12.6%);而受照射24Gy的K562细胞存活率却呈现下降趋势(69.4%、66.1%),不同剂量照射后细胞凋亡率反而增加。细胞周期分析结果显示,C3处理组AHH-1细胞12Gy辐射后G2期阻滞(27.2%)与单纯照射组(40.8%)相比减轻;而K562受照细胞则反而加重。结论 C3对AHH-1细胞具有较好的辐射防护作用,而对K562细胞则表现为抑制细胞增殖,促进辐射诱导的细胞凋亡并加重G2期阻滞。 |
| 英文摘要: |
| Objective To investigate the application prospective of carboxyfullerene C3as a radioprotectant or assistant for tumor radiotherapy. Methods Different concentrations of C3 were incubated with K562 and AHH-1 cell, CCK-8 assay and trypan blue rejection test were performed to examine the influence of C3 on the cell viability. AnnexinV/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after γ-ray irradiation. Results C3 showed little toxicity to AHH-1 cell with the survival rate over 95%, but 600 mg/L of C3 markedly inhibited the growth of K562 cell(82%). Pretreatment of 100 mg/L C3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3%vs 90.3%), but decreased the apoptosis rate(26.3% vs 12.6%), while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C3 concentration. Moreover, the cell cycle analysis revealed the G2 phase block in AHH-1 cell after radiation exposure was mitigated by C3 pretreatment, but that in K562 cell was aggravated. Conclusions C3 has good radioprotective effects on AHH-1 cells. For K562 cell, C3 could inhibit the cell proliferation, promote the radiation induced apoptosis and aggravate the G2 phase block. |
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