| 许杨,王军平,陈芳,申明强,陈默,王崧,冉新泽,粟永萍,开丽.重组融合蛋白rTMP-GH促进放射损伤小鼠 血小板生成的实验研究[J].中华放射医学与防护杂志,2009,29(6):586-585 |
| 重组融合蛋白rTMP-GH促进放射损伤小鼠 血小板生成的实验研究 |
| Effect of rTMP-GH recombinant fusion protein on thrombocytopoiesis in irradiation injured mice |
| 投稿时间：2009-02-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.007 |
| 中文关键词: TPO模拟肽 生长激素 放射损伤 血小板生成 |
| 英文关键词:Thrombopoietin mimetic peptide Growth hormone Irradiation injury Thrombocytopoiesis |
| 基金项目:国家863计划(2007AA02Z152);国家教委高校全国优博论文专项资金(200362);新药创制科技专项(2008ZXJ09003-011) |
| 作者 | 单位 | E-mail | | 许杨 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 王军平 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | wangjunp@yahoo.com | | 陈芳 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 申明强 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 陈默 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 王崧 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 冉新泽 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 粟永萍 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | | | 开丽 | 400038 重庆, 第三军医大学军事预防医学院防原医学教研室、全军复合伤研究所, 创伤、烧伤与复合伤国家重点实验室 | |
|
| 摘要点击次数: 2755 |
| 全文下载次数: 1999 |
| 中文摘要: |
| 目的 研究TPO模拟肽(TMP)与人生长激素(GH)的重组融合蛋白rTMP-GH对放射损伤小鼠血小板生成的促进作用。方法 60Coγ射线全身一次性均匀照射5 Gy,照后皮下注射200 μg／kg 的rTMP-GH或rhGH,每日1次,连续用药7 d,对照组给予同等体积生理盐水。尾静脉取血检测血小板数量变化;照后16 d取小鼠股骨,组织切片观察骨髓病理变化。结果 照后10 d起,rTMP-GH处理组各时相的外周血血小板数明显高于rhGH处理组和生理盐水对照组,尤其是血小板的最低值显著升高( P <0.01),照后22 d已升至正常水平的80%,对照组仅为30%。骨髓病理观察显示,rTMP-GH处理组骨髓造血重建明显,并见大量不同时期巨核细胞;rTMP-GH处理组骨髓组织切片每个视野下的巨核细胞平均数(3.07±0.32)明显高于rhGH处理组(2.20±0.22, P <0.05)和生理盐水对照组(0.87±0.19, P <0.01)。结论 rTMP-GH能快速、有效地促进放射损伤小鼠骨髓巨核细胞增生和血小板生成。 |
| 英文摘要: |
| Objective To investigate the in vivo effects of rTMP-GH recombinant fusion protein on thrombocytopoiesis in mice with thrombopenia inflicted by irradiation. Methods BALB／C mice weighting around 20 g were irradiated with 5 Gy of 60Coγ-ray irradiation to generate thrombopenia. The irradiation injured mice were injected with rTMP-GH or rhGH subcutaneously at the dose of 200 μg·kg－1·d－1 for 7 days. From the 6th day, the platelets in blood samples from vena caudalis were counted routinely, and the pathological changes of bone marrow were determined by morphological observation. Results From the 10th day, the levels of blood platelet in rTMP-GH treated mice were much higher than those of rhGH treatment group and normal saline (NS) control group, especially at the nadir ( P <0.01). On the 22nd day, the platelet count has recovered up to 80% of normal level in rTMP-GH treatment group, while it has just recovered up to 30% in NS control group. Morphological observation showed that there was obvious reconstruction of bone marrow in mice treated with rTMP-GH, compared with NS group.The number of megarkaryoblasts and megakaryocytes in bone marrow of rTMP-GH treated mice (3.07±0.32) was much higher than those of rhGH treatment group (2.20±0.22, P <0.05) and NS control group (0.87±0.19, P <0.01). Conclusions rTMP-GH has potent effects on the recovery of blood platelet by promoting megarkaryocytopoiesis in irradiation injuried mice. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|