| 徐小洁,王凌雪,蔺静,丁丽华,王荣福,秦玺,程龙,康磊,叶棋浓.放射诱导基因Gadd 45在健康人群中表达水平的 检测及其意义[J].中华放射医学与防护杂志,2009,29(6):580-582 |
| 放射诱导基因Gadd 45在健康人群中表达水平的 检测及其意义 |
| Expression of irradiation inducible gene Gadd 45 in population |
| 投稿时间：2009-06-11 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.006 |
| 中文关键词: Gadd 45 生物剂量计 基因表达 |
| 英文关键词:Gadd 45 Biological dosimeter Gene expression |
| 基金项目:全军医药卫生科研基金(06J021);国家重大科学研究计划(2007CB914603) |
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| 中文摘要: |
| 目的 考察Gadd 45基因在健康人群个体间表达的差异,评估Gadd 45发展成为一种新的辐射生物剂量计的可行性。方法 采集20例健康献血员血液样本,提取白细胞RNA进行反转录,以β-actin作为内对照基因,建立实时定量聚合酶链式反应(PCR)方法,并以此方法探讨人群中Gadd 45基因表达水平的差异。结果 20例受试者血液标本中的Gadd 45表达水平之间存在较大差异,且其表达水平与性别无关。结论 放射诱导基因Gadd 45的表达可能不适合做生物剂量计。 |
| 英文摘要: |
| Objective To investigate the difference of Gadd 45 expression in healthy population, and to evaluate the possibility of developing Gadd 45 expression into a new radiation bio-dosimeter. Methods Twenty blood samples from healthy population were collected and the total RNA was extracted from white blood cells and reverse transcribed into cDNA. The method of real-time PCR was used to detect the different level of Gadd 45 expression in human population, with β-actin as an internal control. Results There was a significant difference in Gadd 45 expression among 20 subjects. In addition, the difference was irrelevant to the sex. Conclusion The expression of irradiation-inducible gene Gadd 45 might not be a good bio-dosimeter. |
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