| 王宏,刘强,杨永华,王蕾,姜恩海,樊飞跃.单细胞凝胶电泳检测辐射诱导的DNA双链 断裂修复时效性研究[J].中华放射医学与防护杂志,2009,29(6):564-567 |
| 单细胞凝胶电泳检测辐射诱导的DNA双链 断裂修复时效性研究 |
| Time effectiveness of DNA injury and its repair induced by irradiation using single cell gel electrophoresis |
| 投稿时间：2009-05-25 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.002 |
| 中文关键词: 单细胞凝胶电泳 DNA损伤修复 DNA双链 |
| 英文关键词:Single cell gel electrophoresis DNA damage and repair DNA double-strand break |
| 基金项目:国家自然科学基金(30800281);天津市自然科学基金(07JCYBJC09200) |
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| 中文摘要: |
| 目的 探讨细胞DNA辐射损伤修复时效性,拟合修复曲线和修复平面模型,提高其在辐射生物剂量估算中应用的准确性。方法 通过对离体外周血淋巴细胞用不同剂量γ射线照射后3、24、48 和72 h进行中性单细胞凝胶电泳实验,检测辐射诱导的DNA双链断裂修复程度。结果 照射后不同时间点的剂量-效应曲线和不同剂量点的修复曲线拟合度均较高(r >0.98, P <0.01),将两者结合后,拟合的曲面光滑度较好。结论 不同剂量下的损伤修复呈线性关系,修复曲面模型可用于辐射原发损伤估算。 |
| 英文摘要: |
| Objective To improve the accuracy of estimation of radiation biodosimetry after radiation accident, to explore the time-effect relationship of DNA repair after irradiation, and to describe the curves and plane of DNA repair. Methods The neutral single cell gel electrophoresis (SCGE) was performed to estimate the repair of DNA double-strand break (DNA-DSB) in human lymphocytes induced by different dose of radiation at 3, 24, 48, 72 h post irradiation. Results Better goodness of fit was obtained not only in the dose-response curves at different time points, but also in the time-response curves at different doses. A smooth three-dimensional plane model was obtained when combining the two curves. Conclusions The curves of DNA-DSB repair showed a linear relationship, and repair of radiation-plane model could be used for biological dosimetry. |
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