| 张力元,吴永友,张明芝,等.低剂量辐射诱导的旁效应及其机制的初步研究[J].中华放射医学与防护杂志,2009,29(5):483-485.ZHANG Li-yuan,WU Yong-you,ZHANG Ming-zhi,et al.Low dose radiation induced bystander effect and its mechanism[J].Chin J Radiol Med Prot,2009,29(5):483-485 |
| 低剂量辐射诱导的旁效应及其机制的初步研究 |
| Low dose radiation induced bystander effect and its mechanism |
| 投稿时间：2008-09-10 |
| DOI： |
| 中文关键词: 低剂量辐射 旁效应 兴奋效应 超氧自由基 c-fos |
| 英文关键词:Low dose radiation Bystander effect Hormesis Superoxide radical c-fos |
| 基金项目:苏州市科教兴卫基金资助项目(SWQ19) |
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| 中文摘要: |
| 目的 研究低剂量照射(6cGy)的骨髓细胞悬液,离心后得到的上层相(简称条件液)对正常或辐射损伤细胞能否产生刺激性辐射旁效应,并探讨其发生机制。方法 条件液与受0、2或5 Gy照射的骨髓细胞悬液进行混合培养,使用MTT比色法观察各组细胞的增殖能力。同时采用细胞色素C还原法测定培养介质中O-2的浓度,以及运用免疫组织化学法检测细胞内c-fos的蛋白表达。结果 受大剂量照射的细胞与条件液共培养后,其增殖能力与对照组比较,差异有统计学意义(P<0.01),且伴随着O-2浓度升高和c-fos蛋白表达的上调(P<0.05)。结论 低剂量辐射可对辐射损伤细胞产生促进其增殖的刺激性辐射旁效应,发生机制可能O-2浓度的提高与c-fos蛋白表达的上调有关。 |
| 英文摘要: |
| Objective To investigate whether the supernatant (the conditioned fluid) of myeloid cells suspension after low dose radiation (6 cGy) in vitro could result in hormesis on the normal or radiation damage cells and its mechanism. Methods Mice myeloid cell suspension was irradiated by 0, 2 and 5Gy, respectively, and cultured in vitro. MTT method was used to measure the reproductive activity of cells. Cytochrome C reduction method was used to determine the concentration of O-2, the immunohistochemical method to test the protein expression of c-fos. Results Co-cultured with the conditioned fluid, the reproductive activity of the myeloid cells after high dose irradiation(P<0.01),while both the concentration of O-2 and the protein expression of c-fos were enhanced(P<0.05). Conclusions The conditioned fluid could enhance the proliferation of the myeloid cells after radiation injury. The mechanism of the low dose radiation-induced bystander effect might be correlated with the increase of both the concentration of O－2 and the protein expression of c-fos. |
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