| 章文成,杨晶,杨占山.血管内皮生长因子转基因细胞ECV-304辐射生物效应的研究[J].中华放射医学与防护杂志,2009,29(5):463-467.ZHANG Wen-cheng,YANG Jing,YANG Zhan-shan.Radiobiological effect of VEGF-transfected vascular endothelial cells ECV-304[J].Chin J Radiol Med Prot,2009,29(5):463-467 |
| 血管内皮生长因子转基因细胞ECV-304辐射生物效应的研究 |
| Radiobiological effect of VEGF-transfected vascular endothelial cells ECV-304 |
| 投稿时间：2008-07-28 |
| DOI： |
| 中文关键词: 血管内皮生长因子 血管内皮细胞 辐射损伤 生物效应 |
| 英文关键词:Vascular endothelial growth factor Vascular endothelial cell Radiation injury Biological effect |
| 基金项目:国家自然科学基金(30570549);国防基础科研基金资助项目(A3820060138) |
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| 中文摘要: |
| 目的 通过建立正反义血管内皮生长因子(VEGF)转基因细胞系,研究VEGF对血管内皮细胞ECV-304的辐射生物学特性的影响。方法 鉴定VEGF正义表达载体,并构建其反义载体,建立转染VEGF正反义表达载体的血管内皮细胞系;应用MTT法、微核法研究转染细胞系的辐射生物学变化。结果 将VEGF正反义表达载体成功转染到ECV-304中,建立稳定细胞系。与正常细胞相比,转染正义表达载体的ECV-304稳定细胞系经过4 Gy γ射线照射后,细胞促生长率提高至(10.8±1.9)%(P＜0.05),细胞微核率(MNF)从(53.5±6.61)%减少到(42.5±2.46)%(P＜0.01),微核细胞率(MNCF)从(31.6±5.23)%减少至(23.7±2.27)%(P＜0.05);而转染反义表达载体的细胞系细胞促生长率降低至(-9.2±2.8)%(P＜0.05),MNF、MNCF分别增加至(62.1±1.96)%(P＜0.01)、(38.1±1.47)%(P＜0.05)。结论 VEGF对血管内皮细胞具有辐射防护作用,为今后进一步研究放射性溃疡的转基因治疗提供了实验和理论依据。 |
| 英文摘要: |
| Objective To establish and identify the radiobiological characters of VEGF-transfected cell line. Methods The expression vectors of VEGF165 sense and antisense gene were transfected into vascular endothelial cells. The stable cell line was established and its radiobiological effects were evaluated using MTT method and micronucleus assay. Results The sense and anti-sense expression vectors were successfully transfected into the vascular endothelial cells and the stable cell lines were established. The cell line transfected with sense expression vector of VEGF165 gene showed enhanced proliferation (10.8±1.9)%(P＜0.05). The micronucleus frequency(MNF) was reduced from (53.5±6.61)% to (42.5±2.46)%(P＜0.01),and the micronucleus cell frequency(MNCF) reduced from (31.6±5.23)% to (23.7±2.27)%(P＜0.05). The opposite results were obtained in the anti-sense transfected cell line, which showed the reduction in proliferation(-9.2±2.8)%(P＜0.05),but the increase in MNF(62.1±1.96)%(P＜0.01) and MNCF (38.1±1.47)%(P＜0.05). Conclusions VEGF has a protective effect on the vascular endothelial cells exposed to γ ray irradiation, which may illustrate a new evidence of therapy for radiation injury. |
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