| 张海,王炳胜,刘秀芳,李凤玉,雒书鹏,李宏伟,石军.益气活血中药对大鼠TGF-β1、TNF-α的影响[J].中华放射医学与防护杂志,2009,29(4):383-385 |
| 益气活血中药对大鼠TGF-β1、TNF-α的影响 |
| Effect of Yiqi Huoxue Recipe on the expression of TGF-β1, TNF-α in rats |
| 投稿时间：2009-04-30 |
| DOI： |
中文关键词: 放射性肺损伤 转化生长因子-β1(TGF-β
1) 肿瘤坏死因子-α(TNF-α) 益气活血 |
| 英文关键词:Radiation-induced lung injury TGF-β1 TNF-α Yiqi Huoxue |
| 基金项目:全军医学科学技术研究"十一五"计划课题(06MB084) |
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| 中文摘要: |
| 目的 探讨益气活血中药对小剂量重复照射下不同时相大鼠血清、肺组织中转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)蛋白的表达,探讨其对放射性肺损伤的防治作用及作用机制。方法 66只Wistar雌性大鼠随机表法分为照射加中药组(A组)30只、单纯照射组(B组)30只、健康对照组(C组)6只。6MV直线加速器对前2组大鼠右肺进行分次照射(5 Gy/次,1次/周,累积剂量最高为30 Gy),每组各6只大鼠于照射后第4、6、8、12及26周处死,C组于第4周末处死,取血清标本进行酶联免疫吸附法(ELISA)检测,取肺组织标本进行免疫组织化学检测,观察各组大鼠不同时相血清、肺组织中TGF-β1、TNF-α蛋白表达的动态变化。结果 B组大鼠血清、肺组织中TGF-β1、TNF-α于照射第4周增高,第12周达高峰,第26周略下降;A组各时相大鼠血清、肺组织中TGF-β1、TNF-α蛋白表达趋势与B组相同,但低于B组。结论 益气活血中药具有抑制TGF-β1 、TNF-α合成分泌的作用及减轻放射性肺损伤的作用,其机制与抑制TGF-β1、TNF-α表达有关。 |
| 英文摘要: |
| Objective To observe the effect of Yiqi Huoxue Recipe on the expression of TGF-β1 and TNF-α on serum and lung of rats with radiation-induced lung injury in different radiation time, and discuss the preventative and curative effect and mechanisms of Yiqi Huoxue Recipe. Methods 66 Wistar rats were randomly allocated into irradiation with Yiqi Huoxue Recipe-treated group (A group) irradiation group (B group), and normal control group (C group). The first two groups were irradiated at right hemithorax with a dose of 30 Gy/6 fractions (in 6 weeks). The levels of TGF-β1 and TNF-α on rat serum and lung were measured by ELISA and immunohistochemistry at the end of the 4th, 6th, 8th, 12th, and 26th week after the first irradiation. Results TGF-β1 and TNF-α level on rats serum and lung in irradiation group showed an increase from the 4th week after the first irradiation, peaking at the 12th week, and descending at the 26th week. In A group, the levels of TGF-β1 and TNF-α were significantly lower from the 4th week than those of B group. Conclusions Yiqi Huoxue Recipe could inhibit the excretion of TGF-β1 and TNF-α and might play an important role in the prevention and curation of radiation-induced lung injury, which might be related to inhibiting the expression of TGF-β1 and TNF-α. |
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