| 贺冠强,郑邈,孙汉英,张娜,黄丽芳,王芳,刘文励.辛伐他汀对急性放射损伤小鼠骨髓造血恢复的影响[J].中华放射医学与防护杂志,2009,29(4):372-374 |
| 辛伐他汀对急性放射损伤小鼠骨髓造血恢复的影响 |
| Effects of simvastatin on the recovery of hematopoiesis in acute irradiation injured mice |
| 投稿时间：2008-12-27 |
| DOI： |
| 中文关键词: 辛伐他汀 成骨细胞 放射损伤 骨髓 造血恢复 |
| 英文关键词:Simvastatin Osteoblast Irradiation injury Bone marrow Hematopoietic recovery |
| 基金项目:国家自然科学基金(30570773);高等学校博士学科点专项科研基金(200804871044) |
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| 中文摘要: |
| 目的 探讨小鼠受到照射后,辛伐他汀对骨髓造血功能恢复的影响。方法 将66只8周龄清洁级健康昆明小鼠随机分为3组。正常组不做任何处理;对照组和辛伐他汀组于6.0 Gy 60Coγ线一次性全身均匀照射后, 分别胃饲等体积的生理盐水和辛伐他汀(16 mg/kg,1次/d),直至处死。于照射后第3、7、10、14 天检测小鼠外周血细胞数和骨髓有核细胞数,并用流式细胞仪检测骨髓有核细胞中CD34+细胞百分率,测量骨髓造血面积,观察照射后第10天小鼠脾集落形成单位(CFU-S)数及第28天骨小梁的变化。结果 照射后第3、7、10、14 天辛伐他汀组小鼠外周血白细胞数、血小板数、骨髓有核细胞数和骨髓CD34+细胞率均明显高于对照组(P<0.05或P<0.01),而外周血红细胞数无明显差异。照射后第28天辛伐他汀组小鼠骨小梁的数目明显多于对照组。辛伐他汀组小鼠CFU-S数(11±3)个显著高于对照组(4±3)个。2组小鼠骨髓造血面积随时间的变化差异有统计学意义(P<0.05)。结论 辛伐他汀能够促进急性放射病小鼠成骨细胞增生、增加骨髓CD34+细胞率并促进骨髓造血功能的恢复。 |
| 英文摘要: |
| Objective To investigate the effect of simvastatin on hematopoiesis in acute irradiation injured mice. Method\ Sixty-six Kunming mice were randomly divided into 3 groups as normal group, control group and simvastatin group. Control group and simvastatin group were administrated 0.9% saline and simvastatin(16 mg/kg,1 time each day),respectively after 6.0 Gy 60Co γ-rays. The effects were observed at 3rd, 7th, 10th and 14th day after irradiation by evaluating blood cells, bone marrow nucleated cells, the percentage of CD34+ cells and hematopoietic tissue area. The colony forming units of spleen (CFU-S) at 10th day and bone trabecula at 28 day were calculated. Result The peripheral leukocytes, platelets, the bone marrow mononuclear cells (BMNC) counts, and the percentage of CD34+ cells in the bone marrow at each tested time were increased significantly after simvastatin treatment (P<0.05 or P<0.01). The number of bone trabecula at 28th day and CFU-S counts at 10th day in simvastain group were also increased significantly. ConclusionsSimvastatin could accelerate the early phase of hematopoietic recovery in irradiation injured mice by increasing the number of bone trabecula, osteoblasts and CD34+ cells in bone marrow. |
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