| 崔玉芳,姜竺君,杜丽,马琼,柳晓兰,谢玲,董波,毛建平,满其航,张超.γ线照射小鼠胸腺组织基因表达转录谱分析[J].中华放射医学与防护杂志,2009,29(4):355-359 |
| γ线照射小鼠胸腺组织基因表达转录谱分析 |
| Analysis of the transcription profiles of mouse thymus irradiated by γ-rays |
| 投稿时间：2008-12-18 |
| DOI： |
| 中文关键词: 电离辐射 胸腺 基因芯片 细胞周期 免疫应激 凋亡 |
| 英文关键词:Ionizing radiation Thymus Gene chips Cell cycle Immune stress Apoptosis |
| 基金项目:国家科技重大专项 |
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| 摘要点击次数: 3072 |
| 全文下载次数: 2015 |
| 中文摘要: |
| 目的 探讨6 Gy γ线照射后不同时间小鼠胸腺组织基因表达转录谱的动态变化。方法 应用快速高通量的cDNA基因芯片技术进行研究。结果 1随照射后时间推移,差异表达基因数目和种类逐渐减少,如照射后1、6、14和28 d差异基因数量分别为878、318、162和25;2辐射所诱导的胸腺组织差异表达基因广泛涉及细胞周期、免疫和应激、细胞凋亡、细胞信号转导、转录调节、DNA合成修复和重组、细胞骨架、离子通道和运输、代谢、蛋白翻译和合成、发育、细胞分化多个方面;3照射后对某些重要差异表达基因的分析表明,与细胞周期相关的基因有5个(上调3个:Cyclin G、Anxa1、Fgf1,下调2个:Cdc2a、Cdc25b);与免疫应激相关的5个(上调4个:IL-18、Casp1、IL-15、IL-7,下调1个:Cd28);与细胞凋亡相关的7个(上调4个:Casp1、Anxa1、Perp、IL-7,下调3个:Pten、Api5、Fas)。结论 6 Gy γ线照射后,小鼠胸腺组织差异表达基因随照射后时间延长不仅涉及多个靶点、多个层次,显示出多样性变化的特点,而且显示出时间上的明显差异;从基因水平上揭示了中等剂量照射小鼠胸腺组织损伤修复和重建基因的变化规律,为辐射免疫损伤与修复的分子机制和防治措施的研究提供了新的启示。 |
| 英文摘要: |
| Objective To investigate the dynamic changes of transcription profiles of mouse thymus gene expression in different times after 6 Gy γ-irradiation. Methods High-flux cDNA microarray technique was used to detect the changes in gene expression of irradiated mouse thymus. Results 1 With time-lapse after irradiation, the numbers and types of differentially expressed genes were gradually decreased, for instance, the gene numbers were 878, 318, 162 and 25 at 1, 6, 14 and 28 d after irradiation, respectively. 2Radiation-induced differential expression genes were involved in cell cycle, immunity and stress, apoptosis, signal transduction, transcription regulation, DNA synthesis and recombination, cystoskeleton, ion channel and transportation, metabolism, protein translation and synthesis, development and cell differentiation, etc. 3 Analysis of some important differential expression genes after irradiation showed that there were 5 genes correlated cell cycle (3 up-regulating:Cyclin G,Anxa1,Fgf1 and 2 down-regulating:Cdc2a, Cdc25b), 5 genes correlated immune stress(4 up-regulating:IL-18, Casp1, IL-15, IL-7 and 1 down-regulating:Cd28), 7 genes correlated apoptosis(4 up-regulating:Casp1, Anxa1, Perp, IL-7 and 3 down-regulating:Pten, Api5 and Fas). Conclusions After 6 Gy irradiation, differentially expressed genes in mouse thymus is not only involved in many targets, levels and pathways, but also displayed an obvious difference in times. This reveals the regular pattern of differential expression genes in the process of injury and reconstitution in moderate dose irradiated mouse thymus. |
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