| 商希梅,陈英民,毕明卫,宋钢,马娅,李贞,乔建维.金矿井下工作人员细胞遗传学调查分析[J].中华放射医学与防护杂志,2009,29(2):201-203 |
| 金矿井下工作人员细胞遗传学调查分析 |
| Cytogenetic investigertion on underground workers in gold mine |
| 投稿时间：2009-01-07 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.028 |
| 中文关键词: 金矿 染色体畸变 CB微核 职业危害 |
| 英文关键词:Gold mine Chromosome aberrations Cytokinesis_block micronucleus Occupational hazards |
| 基金项目:科技部科研院所社会公益研究专项(2005DIB1J087) |
|
| 摘要点击次数: 3064 |
| 全文下载次数: 1786 |
| 中文摘要: |
| 目的 探讨某金矿井下凿岩等从业人员外周血淋巴细胞染色体畸变与微核情况。方法 采用微量全血培养法和胞质分裂阻滞(CB)法,分析调查对象的染色体畸变与微核细胞形成情况。结果 井下从业人员染色体型畸变率为0.72%,染色单体型畸变率为0.41%,总畸变率为1.16%,淋巴细胞微核细胞率为10.8‰,微核率为11.6‰,均明显高于健康对照组(0.14%、0.18%、0.33%、8.7‰和9.0‰),差异有统计学意义(χ2=44.322、9.501、50.476、8.672、12.546,P<0.01)。染色体畸变率与微核阳性检出率随着井下工龄的增加而呈升高的趋势,各工龄组间和健康对照组比较,差异有统计学意义(P<0.01)。井下从业21年以上工龄组明显高于井下从业6年以下工龄组,但其他各工龄组间差异无统计学意义(P>0.01)。染色体畸变类型主要以无着丝粒断片为主,其次是双着丝粒体和单体断裂,偶见一个细胞内有两种畸变。结论 目前金矿井下凿岩等从业者存在一定的职业危害效应,应引起相关部门的高度重视。 |
| 英文摘要: |
| Objective To investigate the chromosome damage in peripheral lymphocytes of underground gold miners.Methods Conventional method and cytokinesis_block micronucleus assay were used to analyze frequency of chromosome aberrations and micronucleus in peripheral lymphocytes in 58 gold miners, respectively.Results Frequencies of chromosome-type aberrations, chromatid-type aberrations and total aberrations were higher in the miners than those in the control group (0.72%, 0.41%,1.16% vs 0.14%, 0.18%,0.33,χ2=44.322,9.501,50.476,P<0.01). Both micronucleated cell rate and micronucleus rate were higher in the miners group than those in the control group (10.8‰ and 11.6‰ vs 8.7‰ and 9.0‰,χ2=8.672,12.546,P<0.01).Frequencies of chromosomal aberrations and micronucleus proportionally increased with underground working years. Compared with those miners who had worked underground 6 years or shorter, both frequencies were statistically higher among the miners who had worked underground for more than 21 years (P<0.05). No difference was found among other groups of working years (P>0.05). Compared with the control group, both frequencies increased in the miner group, and the differences were statistically significant (χ2=2.395, P<0.05 for chromosomal aberrations and χ2=2.319,P<0.05, respectively). The common types of chromosome aberrations were acentric fragments, while chromatid break and dicenrics were subordinate.Conclusions Chromosomal damages were observed in the gold workers who exposed high radon in the underground mining. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|