| 齐雪松,郝佐红,吕慧敏,王春燕,张伟,王平,郝述霞,苏旭.含钍稀土粉尘致TNF-α、Il-2和Il-6基因多态性对肺通气功能的影响[J].中华放射医学与防护杂志,2009,29(2):199-200 |
| 含钍稀土粉尘致TNF-α、Il-2和Il-6基因多态性对肺通气功能的影响 |
| Correlation between gene polymorphisms of IL-2, IL-6 and TNF-α in workers exposed to rare earth dust and their lung ventilation function |
| 投稿时间：2009-01-09 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.027 |
| 中文关键词: IL-2 IL-6 TNF-α 基因多态性 肺通气功能障碍 |
| 英文关键词:IL-2 IL-6 TNF-α Gene polymorphism Lung ventilation dysfunction |
| 基金项目:科技部科研院所社会公益研究专项(2005DIB1J087) |
|
| 摘要点击次数: 2745 |
| 全文下载次数: 1562 |
| 中文摘要: |
| 目的 通过对暴露于稀土粉尘工作人员外周血细胞的IL-2、IL-6和TNF-α基因多态性位点的检测,对接尘工作人员的肺通气功能进行初步探讨。方法 TNF-α(-308,G→A) 基因多态性检测采用基因体外扩增限制性片段长度多态性分析方法,IL-2(-330, T→G)和IL-6(-174G→C)基因多态性均采用相对的两条引物聚合酶链反应检测。结果 接尘组与非接尘组相比较,TNF-α基因多态性(χ2=4.03,P>0.05)、IL-2基因多态性(χ2=2.21,P>0.05)和IL-6基因多态性(χ2=1.05,P>0.05)差异均无统计学意义。IL-2基因突变纯合型致肺通气功能障碍的发病风险是野生型的4.29倍(95%可信限1.09~16.9)。结论 IL-2基因突变纯合型更易加重肺部炎症反应。接尘组人员的肺通气功能障碍发生率与非接尘组的相比有增多的趋势。 |
| 英文摘要: |
| Objective To study the relationship between lung ventilation function of workers exposed to rare earth dust and their IL-2, IL-6 and TNF-α gene polymorphism. Methods TNF-α gene polymorphism were identified by RFLP-PCR, IL-2 and IL-6 gene polymorphisms were identified by PCR-CTPP analysis. Lung ventilation function was deteced by instrument of ventilation function. Results Compared with controls, there was no statistic significance in frequencies distribution of TNF-α gene polymorphism (χ2=4.03,P>0.05), IL-2 gene polymorphism (χ2=2.21,P>0.05) and IL-6 gene polymorphism (χ2=1.05,P>0.05). Compared with IL-2 gene wild type, IL-2 homozygote type increased the risk of lung ventilation dysfunction by 4.29 folds (95% CI 1.09~16.9). Conclusion Compared with controls, incidence of ventilation function of workers exposed to rare earth dust is in ascending trend. IL-2 (G/G) gene type induces more serious inflammation reaction than the others. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|