| 杨焕,徐国旭,刘东伟,刘玉龙,卜曙,季晓燕.αB-晶体蛋白在放射性白内障大鼠眼晶状体中的表达[J].中华放射医学与防护杂志,2009,29(2):160-162 |
| αB-晶体蛋白在放射性白内障大鼠眼晶状体中的表达 |
| Expression of αB-crystallin in X-ray irradiated rat lenses |
| 投稿时间：2008-10-06 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.011 |
| 中文关键词: αB-晶体蛋白 放射性白内障 分子伴侣 |
| 英文关键词:αB-crystallin Radiation-induced cataract Molecular chaperone |
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| 中文摘要: |
| 目的 检测受不同剂量X射线照射的大鼠晶状体中可溶性αB-晶体蛋白的表达水平,探讨αB-晶体蛋白与放射性白内障形成之间的关系。方法 用直线加速器X射线一次性照射SD雄性大鼠双眼,建立放射性白内障模型。将大鼠分为健康对照组、实验对照组和5、15及25 Gy照射组。于照射后3个月将大鼠处死,取晶状体作匀浆,取其上清液(可溶性晶状体蛋白质)采用免疫印迹法(Western blot) 检测αB-晶体蛋白的表达。结果 健康对照组、实验对照组和5 Gy组未出现晶状体混浊,15和25 Gy的照射组逐渐形成典型放射性白内障。蛋白免疫印迹结果显示随着照射剂量的增加,SD大鼠眼晶状体内可溶性αB-晶体蛋白逐渐减少,差异有统计学意义(F=40.764,P<0.05) 。结论 在放射性白内障中晶状体内可溶性αB-晶体蛋白表达减少,在白内障形成中起重要作用。 |
| 英文摘要: |
| Objective To detect the changes of soluble αB-crystallin in X-ray irradiated rat lens, and to explore the potential role of αB-crystallin in the pathogenesis of radiation-induced cataract.Methods The radiation cataract model was established with the accelerator linear in male Sprague-Dawley (SD) rats. The rats were divided into normal control group, experimental control group and X-irradiated group (the doses were 5, 15, and 25 Gy, respectively). The rats were killed at 3 months post-irradiation, and the lenses were carefully isolated and homogenized. The protein changes of αB-crystallin in lens supernatant were measured by Western blotting.Results Typical radiation-induced cataract was observed in 15 and 25 Gy groups, while the lenses of the normal control group, experimental control group and the 5 Gy irradiation group remained transparent. The αB-crystallin protein level was significantly decreased in a dose-dependent manner (5 Gy group:0.871±0.085;15 Gy group:0.643±0.096;25 Gy group:0.338±0.160;F=40.764, P<0.05).Conclusion The decreased expression of the molecular chaperone αB-crystallin in X-irradiated rat lens indicates its important role in the pathogenesis of irradiation cataract. |
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