| 庄洪卿,王俊杰.EGFR抗体对持续低剂量率照射CL187细胞的放射增敏研究[J].中华放射医学与防护杂志,2009,29(2):157-159 |
| EGFR抗体对持续低剂量率照射CL187细胞的放射增敏研究 |
| EGFR antibody radiosensitivity enhancement on CL187 cancer cells after continous low dose rate irradiation |
| 投稿时间：2008-02-05 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.010 |
| 中文关键词: 低剂量率照射 125I粒子 表皮生长因子 放射增敏 |
| 英文关键词:Low_dose_rate irradiation 125I seed EGFR Radiosensitivity |
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| 摘要点击次数: 2771 |
| 全文下载次数: 1997 |
| 中文摘要: |
| 目的 探讨表皮生长因子受体(EGFR)抗体对放射性125I粒子持续低剂量率照射大肠癌细胞CL187的增敏作用。方法 采用CL187大肠癌细胞系体外培养,实验分空白对照组、单纯抗体组、单纯照射组、照射加不同浓度抗体组。用克隆形成方法评价不同处理方式对CL187细胞的杀伤效应。应用流式细胞仪检测细胞凋亡和周期的改变。结果 EGFR与射线联合作用细胞存活分数较单纯照射组明显下降(P<0.05)。EGFR抗体浓度2、5、10 nmol/L时增敏比分别为1.34、1.59、2.08。持续低剂量率照射4 Gy后,抗体联合照射组凋亡率(39.86%±4.38%)高于单纯抗体组(12.49%±1.59%)和单纯照射凋亡率之和(21.57%±2.97%),差异有统计学意义(P<0.05)。细胞周期检测显示表皮生长因子抗体联合照射主要引起细胞的G1期阻滞(84.51%±3.42%),持续低剂量率照射主要引起G2/M期阻滞(46.41%±4.48%),两者联合作用时,G1期(59.31%±5.34%)和G2/M期(38.10%±2.57%)细胞比率增加,S期(2.59%±1.19%)细胞比率明显减少(P<0.05)。结论 表皮生长因子抗体对持续低剂量率照射具有明显的放射增敏作用,其增敏机制主要来自细胞周期的重分布和细胞凋亡的增加。 |
| 英文摘要: |
| Objective To investigate the radiosensitivity enhancement on CL187 cancer cell lines after continous low_dose_rate irradiation of 125Iseeds blocked by EGFR antibody.Methods There were control group, the irradiation or plus EGFR antibody group, with 3 samples in each group. Clongenic assay was used to detect the survival of cells.The cell cycle distribution and apoptosis were analyzed by flow cytometry.Results The survival fraction of CL187 cells were lower after blocked by EGFR antibody at the same dose irradiation. The SER were 1.34,1.59 and 1.98 when the antibody concentration were 2,5 and 10 nmol/L,respectively.The irradiation plus antibody led to more apoptosis(39.86%±4.38%) of CL187 cells than the irradiation (21.57%±2.97%) plused antibody(12.49%±1.59%) worked alone. Comparison of the low dose rate irradiation related to G2/M cell cycle arrest(46.41%±4.48%), More G1 cell cycle arrest(84.51%±3.42%)walked together along with the EGFR antibody.Conclusion EGFR antibody could enhance the cell radiosensitivity after continuous low_dose_rate irradiation. The cell cycle redistribution and apoptosis might be the main mechanism of the radiosensitivity enhancement. |
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