| 保鹏涛,戚好文,高维,李树钧,张力江.乌司他丁对大鼠放射性肺损伤中TNF-α及IL-6的影响[J].中华放射医学与防护杂志,2009,29(2):154-156 |
| 乌司他丁对大鼠放射性肺损伤中TNF-α及IL-6的影响 |
| Effect of ulinastatin on tumor necrosis factor and interleukin-6 in rats with radiation-induced lung injury |
| 投稿时间：2008-04-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.009 |
| 中文关键词: 乌司他丁 肿瘤坏死因子α 白介素6 肺 放射 |
| 英文关键词:Ulinastatin TNF-α IL-6 Lung Radiation |
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| 中文摘要: |
| 目的 观察乌司他丁(天普洛安)对大鼠放射性肺损伤过程中TNF-α及IL-6的影响,试图寻找一种预防或治疗放射性肺损伤的有效途径。方法 72只雌性SD大鼠随机分为3组:健康对照组、单纯照射组和乌司他丁治疗组,每组24只。照射组及治疗组动物麻醉后,行直线加速器全胸部照射一次,剂量为25 Gy。照射组照射后通过尾静脉注射乌司他丁(100?000 U·kg－1·d－1),共计7 d。对照组和照射组注射同等体积的生理盐水。于照射后2 h、4、8和24周处死动物,取部分肺组织行HE染色及Masson染色,观察组织学变化,另提取组织蛋白用免疫印迹杂交方法检测肺组织中TNF-α水平,使用酶联免疫吸附分析法检测血清中IL-6水平。所有数据采用SPSS统计软件进行方差分析。结果 照射组大鼠肺组织中TNF-α及血清中IL-6水平与对照组相比,明显升高,并在第4周时达到顶峰(q=5.63、6.21,P<0.01);治疗组TNF-α和IL-6水平与对照组相比,第4周时也增加明显,但是与照射组相比明显下降(q=4.97、7.42,P<0.01)。结论 大鼠放射性肺损伤时TNF-α及IL-6水平明显升高,在放射性肺损伤发展中起到重要作用,乌司他丁能显著降低其水平,并抑制炎性反应,从而能有效地防治放射性肺损伤。 |
| 英文摘要: |
| Objective To observe the effect of ulinastatin on tumor necrosis factor(TNF-α) and interleukin-6(IL-6)in radiation-induced lung injury.Methods Seventy-two female SD rats were randomly divided into 3 groups as control group,irradiation group and treatment group (administered with Ulinastatin).Rats in irradiation group and treatment group were irradiated with linear accelerator at a single dose of 25 Gy.After irradiation rats in treatment group were injected daily with ulinastatin at a dose of 100 000 U·kg－1·d－1 for 7 days through caudal vein while rats in control group and irradiation group were injected with the same volume of saline. Rats were killed at 2 h,4,8 and 24 weeks. Samples of lung tissues were observed by using HE staining.Expression of TNF-α in lung was determined by Western blot and expression of IL-6 in serum was determined by ELISA. Data were analyzed by SPSS software.Results Expressions of TNF-α in lung and IL-6 in serum increased significantly after irradiated in irradiation group compared with control group, and it reached the peak at 4 weeks(q=5.63、6.21,P<0.01).Though expressions of TNF-α and IL-6 in treatment group also increased compared with control group,the difference between irradiation group and treatment group was statistic significantly (q=4.97、7.42,P<0.01).Conclusion TNF-α and IL-6 play an important role in radiation-induced lung injury. Ulinastatin could suppress the inflammatory response and radiation-induced lung injury effectively by decreasing the levels of TNF-α and IL-6. |
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