| 黄波,陈锋,杨陟华,潘秀颉,曹珍山,朱茂祥.贫铀对BEAS-2B细胞的氧化损伤及二甲基亚砜的保护作用[J].中华放射医学与防护杂志,2009,29(2):143-146 |
| 贫铀对BEAS-2B细胞的氧化损伤及二甲基亚砜的保护作用 |
| Oxidative damage of BEAS-2B cells induced by depleted uranium and protection by DMSO |
| 投稿时间：2008-06-26 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.02.006 |
| 中文关键词: 贫铀 BEAS-2B细胞 活性氧 抗氧化酶系 二甲基亚砜 |
| 英文关键词:Depleted uranium BEAS-2B cell Reactive oxygen species Antioxidant enzyme DMSO |
| 基金项目:国家自然科学基金(30271524);全军十五指令性课题(L061) |
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| 摘要点击次数: 3138 |
| 全文下载次数: 2011 |
| 中文摘要: |
| 目的 观察贫铀(DU)诱发细胞氧化损伤和二甲基亚砜(DMSO)的保护作用。方法 以人支气管上皮细胞(BEAS-2B)为靶细胞,分别用辣根过氧化物酶介导的酚红氧化法、细胞色素C还原法和番红花红褪色法测定细胞外过氧化氢(H2O2)、超氧阴离子(O2-·)和羟自由基(·OH)含量;细胞内H2O2和O2-·分别用2,7'-二氯荧光黄双乙酸盐(DCFH2DA)和氢化乙锭(HE)标记,荧光法测定荧光产物2,7'-二氯荧光黄(DCF)和溴乙锭(EB)荧光强度。化学发光法检测SOD活力,分光光度法检测谷胱甘肽活力。结果 BEAS-2B细胞经DU染毒后,细胞内和细胞外ROS含量显著增高;细胞SOD活力及GSH活力下降,DMSO对贫铀诱发的ROS增高及SOD、GSH活力的降低有明显的抑制作用。结论 贫铀可造成细胞的氧化损伤,DMSO通过清除活性氧而达到保护作用,可为DU损伤的防护提供有效措施。 |
| 英文摘要: |
| Objective To observe the oxidative damage in human bronchial epithelial cells (BEAS-2B) induced by depleted uranium (DU) and protection of DMSO.Methods The measurement of extracellular superoxide anions (O2-·) was based on the reduction of ferricytochrome C. Quantitative analysis of extracellular hydrogen peroxides (H2O2) was used by the horseradish peroxidase-dependent oxidation of phenol red. The determination of extracellular hydroxyl radicals (·OH) was based on discoloration of safranine T. Ethidium bromide and 2,7'-dichlorofluorescein, fluorescent products of the membrane-permeable dyes-hydroethineand 2,7'-dichloroflurescin diacetate were used to monitor the intracellular production of O2-· and H2O2 by fluorometric method. The enzyme activity of SOD and GSH were measured by chemiluminescence and spectrophotometric method, respectively.Results The ROS production, including H2O2, O2-· and ·OH, increased remarkably which induced by DU in BEAS-2B cells. The enzyme activity of SOD and GSH was descended remarkedly. These changes could be effectively inhibited by 0.5% of DMSO.Conclusion DU causes oxidative damage to BEAS-2B cells.Through removing active oxygen,DMSO can inhibit oxidative damage of DU. |
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