| 万建美,范我,张友九,朱然,俞泽阳.125I-脱氧尿嘧啶核苷对淋巴瘤细胞Raji和Daudi的杀伤作用[J].中华放射医学与防护杂志,2009,29(1):50-53 |
| 125I-脱氧尿嘧啶核苷对淋巴瘤细胞Raji和Daudi的杀伤作用 |
| Killing effect of 125I-UdR on human lymphoma Raji and Daudi cell lines |
| 投稿时间：2008-06-08 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.015 |
| 中文关键词: 淋巴瘤细胞 Raji Daudi 125I-UdR 摄取 杀伤效应 |
| 英文关键词:Lymphoma cells Raji Daudi 125I-UdR Uptake Killing effect |
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| 中文摘要: |
| 目的 探讨125I-脱氧尿嘧啶核苷(125I-UdR)在淋巴瘤细胞Raji和Daudi中的特异性摄取及其杀伤效应。方法 测量Raji、Daudi细胞和细胞核在含不同放射性浓度125I-UdR的培养液中培养不同时间后的活度;用噻唑蓝(MTT)实验和碘化丙啶(PI)染色周期分析评价125I-UdR对Raji和Daudi细胞的杀伤作用。结果 Raji和Daudi细胞摄取125I-UdR的量明显高于Na125I对照组(P<0.05),在100kBq/ml浓度时,Raji和Daudi细胞摄取125I-UdR的量分别为(14 414±95)和(6916±53.69)Bq/106细胞,而对Na125I的摄取量分别为(68±3.8)和(324±32.8) Bq/106细胞;细胞和细胞核中125I-UdR的量随培养基中125I-UdR放射性浓度以及培养时间的增加而增加;125I-UdR组的存活分数明显低于Na125I对照组(P<0.05),以500kBq/ml浓度培养48h时, Raji和Daudi细胞125I-UdR组的存活分数分别为(19.78±1.39)%和(43.17±2.69)%,而Na125I组的存活分数分别为(79.10±1.79)%和(80.36±6.12)%;细胞存活分数有随培养基中放射性浓度增加而降低的趋势。结论 125I-UdR可被Raji和Daudi细胞特异性摄取并进入细胞核中,进而杀死细胞,其作用具有明显的时间-效应和剂量-效应关系。 |
| 英文摘要: |
| Objective To evaluate the killing effect and the uptake of 125I-UdR on human lymphoma Raji and Daudi cell lines. Methods The amount of 125I-UdR in the cells and cell nuclei were determined after incubation of different time in RPMI 1640 culturing medium containing different concentrations of 125I-UdR. The killing effects of 125I-UdR on Raji and Daudi cell lines were estimated through MTT assay and cell cycle was analyzed by propidium iodide (PI) staining. Results The amounts of 125I-UdR in Raji and Daudi cells and cell nuclei were much higher than that of Na125I(P<0.05). The amounts of 125I-UdR in Raji and Daudi cells were 14 414±95 and (6916±53.69) Bq/106cell when the concentration was 100 kBq/ml. The amounts of Na125I were 68±3.8 and (324±32.8) Bq/106cell. The uptake of 125I-UdR in Raji and Daudi cells and cell nuclei increased with the 125I-UdR concentration and incubated time. The cell surviving fractions of 125I-UdR groups was much lower than that of Na125I groups (P<0.05). When the concentration was 500 kBq/ml and incubated time was 48 hours, the Raji and Daudi cell surviving fractions of 125I-UdR groups were (19.78±1.39)% and (43.17±2.69)%; those of Na125I groups were(79.10±1.79)% and (80.36±6.12)%. The surviving fractions of 125I-UdR groups reduced with the 125I-UdR concentration.Conclusion 125I-UdR can be specially ingested by Raji and Daudi cells and incorporated into DNA, then the cells will be killed. The uptake of 125I-UdR is dose and time dependent. |
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