| 夏新舍,折虹,马建萍,叶红强,丁喆,余文军.3-氨基苯甲酰胺对人食管癌细胞放射增敏作用的实验研究[J].中华放射医学与防护杂志,2009,29(1):46-50 |
| 3-氨基苯甲酰胺对人食管癌细胞放射增敏作用的实验研究 |
| Study on 3-aminobenzamide enhancing the radiosensitivity of human esophageal carcinoma cell strain in vitro |
| 投稿时间：2008-10-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.014 |
| 中文关键词: 食管癌细胞株 放射增敏剂 多聚(ADP-核糖)聚合酶 抑制剂 3-氨基苯甲酰胺 |
| 英文关键词:Esophageal carcinoma cell strain Radiosensitizer Poly(ADP-ribose) polymerase(PARP) Inhibitor 3-Aminobenzamide(3-AB) |
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| 中文摘要: |
| 目的 探讨多聚(ADP-核糖)聚合酶(PARP)抑制剂3-氨基苯甲酰胺(3-AB)对人食管癌细胞株CaEs-17的放射增敏作用及其机制。方法 利用人食管癌细胞CaEs-17为靶细胞, 给予0、2.5、7.5 mmol/L 3-AB及0、3、6、9、12Gy照射剂量, 采用集落形成法及噻唑蓝(MTT)法观察3-AB的放射增敏效应;彗星电泳法、中期染色体分析法分别观察3-AB对放射所致细胞DNA断裂及细胞染色体畸变的影响。结果 根据多靶单击数学模型拟合细胞存活曲线, 得出2.5、7.5mmol/L 3-AB作用于CaEs-17细胞24h的放射增敏比分别为1.21和1.52;MTT实验结果显示3-AB可降低放射细胞的存活分数;3-AB可增加放射所致细胞DNA断裂程度以及放射所致细胞染色单体断裂数。结论3-AB对体外培养人食管癌细胞株CaEs-17有一定放射增敏效应;放射增敏的机制可能与3-AB抑制 PARP的功能从而降低细胞对放射所致DNA断裂的修复水平有关。 |
| 英文摘要: |
| Objective To evaluate the ability of 3-AB to sensitize the human esophageal carcinoma cell strain (CaEs-17) to radiation in vitro and its mechanisms. Methods CaEs-17 cells were treated with 3-AB at 0, 2.5, 7.5 mmol/L and given irradiation 0, 3, 6, 9, 12 Gy. 3-AB concentration in each group was made dose-survival curve using multi-target single-hit maths model by clonogenic assay. MTT assay was performed to observe the survival of irradiated cells. Comet assay and metaphase chromosome analysis were used to measure the DNA damage degree and chromosome aberration of CaEs-17 cell after 3-AB treatment and irradiation. Results Cell survival experiments showed SER of 1.21, 1.52 for 2.5 mmol/L, 7.5 mmol/L 3-AB respectively using multi-target single-hit maths model. The survival fraction of irradiated CaEs-17 cell was decreased after 3-AB treatment. DNA damage and the chromatid breakage number of irradiated CaEs-17 cells were increased after 3-AB treatment.Conclusion 3-AB, a PARP inhibitor, can enhance the radiosensitivity of human esophageal carcinoma cell strain (CaEs-17). DNA damage repair inhibition by 3-AB might be one of the mechanisms. |
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