| 杨青山,王勇,樊飞跃.Ku80对人食管癌细胞放射敏感性及细胞周期的影响[J].中华放射医学与防护杂志,2009,29(1):44-46 |
| Ku80对人食管癌细胞放射敏感性及细胞周期的影响 |
| Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells |
| 投稿时间：2008-03-17 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.013 |
| 中文关键词: shRNA Ku80 辐射敏感性 细胞周期 |
| 英文关键词:shRNA Ku80 Radiosensitivity Cell cycle |
| 基金项目: |
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| 中文摘要: |
| 目的 利用shRNA抑制Ku80蛋白的表达来研究Ku80在食管癌治疗中的作用。方法采用RNA干扰技术,构建shRNA-Ku80载体。用Western blot和RT-PCR方法证实RNA干扰的有效性和可行性。用克隆形成实验来研究shRNA抑制Ku80表达对γ线的敏感性。用流式分析法来研究shRNA抑制Ku80表达对细胞周期的影响。结果 成功构建了shRNA-Ku80载体。shRNA抑制Ku80表达增强食管癌细胞对γ线的敏感性。shRNA K3和H2使细胞周期阻滞于G2/M期(61.8%与28.6%;64.3%与28.6%)。结论 shRNA抑制Ku80表达在食管癌治疗中有作用,有望成为肿瘤治疗的新靶点。 |
| 英文摘要: |
| Objective To study the role of Ku80 in the treatment of esophageal cancer through inhibiting the Ku80 expression by shRNA. Methods shRNA-Ku80 vector was constructed. The effectiveness and feasibility of RNA interference were confirmed by Western blot and RT-PCR methods. The cell sensitivity to gamma-rays was studied by colony formation assay. The effects of shRNA-Ku80 on cell cycle were observed by flow cytometry analysis. Results ShRNA-Ku80 vector was constructed successfully. Inhibition of Ku80 expression by shRNA enhanced the sensitivity of esophageal cancer cells to gamma-rays. shRNA K3 or shRNA H2 showed higher percentage of cells in G2/M phase (61.8% vs 28.6%; 64.3% vs 28.6%). Conclusion Inhibitions of Ku80 expression by shRNA play a role in the treatment of esophageal cancer. Ku80 might be a new target of tumor treatment. |
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