| 郑辉,涂序珉,甄荣,张志兴.单次和累积照射对大鼠抗氧化能力和抗氧化酶活性的影响[J].中华放射医学与防护杂志,2009,29(1):41-43 |
| 单次和累积照射对大鼠抗氧化能力和抗氧化酶活性的影响 |
| Effects of single and accumulative irradiation on ability of antioxidation and the antioxidase activity on rats |
| 投稿时间：2008-08-28 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.012 |
| 中文关键词: 累积照射 一氧化氮 总抗氧化能力 超氧化物歧化酶 谷光甘肽过氧化物酶 过氧化氢酶 丙二醛 |
| 英文关键词:Accumulative irradiation Nitric oxide Total ability of anti-oxidize antioxidase Superoxide dismutase Glutathion peroxidase Catalase Maleic dialdehyde |
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| 中文摘要: |
| 目的 比较单次照射和累积剂量照射对大鼠血清一氧化氮(NO)、总抗氧化能力、抗氧化酶和丙二醛(MDA)含量改变。方法 48只SD 大鼠随机分为单次照射组和累积剂量照射组。采用60COγ线诱发单次和累积剂量照射模型。总照射剂量分别为0.4、0.8、1.6、3.2、6.4Gy。末次照射后1d 检测大鼠血清NO,总抗氧化能力、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性和MDA含量。结果 累积照射后血清NO含量(52.6~117.9 μmol/ml),总抗氧化能力(3.3~26.2 U/ml)、SOD(26.3~167.5 U/ml)、GSH-PX(740.8~2462.4 U/ml)、CAT 活性(3.3~29.4 U/ml)和MDA含量(29.3~155.1 nmol/ml)与单次照射组比较均有不同程度的增加,其变化幅度与累积受照总剂量有关。其中累积照射0.4Gy组总抗氧化能力(26.2 U/ml)、SOD(167.5 U/ml)和CAT 活性(29.4 U/ml)明显大于健康对照组水平,累积受照总剂量>3.2Gy组血清MDA含量明显高于单次照射组,可能与血清中抗氧化酶活性较高有关。结论 累积低剂量电离辐射可诱发生物氧化酶的刺激或兴奋效应,累积高剂量电离辐射对机体抗氧化酶活性仍有明显的损伤作用。 |
| 英文摘要: |
| Objective To compare the changes in nitric oxide (NO), total ability of anti-oxidize antioxidase, superoxide dismutase(SOD), glutathion peroxidase(GSH-PX), catalase(CAT)and maleic dialdehyde(MDA) in rats after single and accumulative 60Co γ-irradiation . Methods 48 rats were randomly divided into two groups of single and accumulative irradiation. Each group was irradiated by 60Co gamma-rays. The total irradiation doses were 0.4, 0.8, 1.6, 3.2 and 6.4 Gy, respectively in each group. The NO, total ability of anti-oxidize antioxidase, SOD, GSH-PX, CAT and MDA in serum were measured at day 1 after last irradiation.Results Compared with the single irradiation group, the NO (52.6-117.9μmol/ml), total ability of anti-oxidize antioxidase (3.3-26.2 U/ml), the antioxidase activity of the SOD (26.3-167.5 U/ml), GSH-PX (740.8-2462.4 U/ml), CAT (3.3-29.4 U/ml) and the content of MDA(29.3-155.1 nmol/ml) of rat serum in accumulative irradiation group were increased after irradiation, which was related with the accumulative irradiation dose. For instance, total ability of anti-oxidize antioxidase (26.2 U/ml), antioxidase activity of the SOD (167.5 U/ml) and CAT (29.4 U/ml) in 0.4 Gy group of accumulative irradiation were significantly increased when compared with those of control group. However, the content of MDA in accumulative irradiation group was obviously higher than that in single radiation group when the irradiation doses delivered over 3.2 Gy, which might be correlated with higher antioxidase activity. Conclusion Low dose of accumulative gamma-rays irradiation can induce the stimulative effect of antioxidase activity. However, higher dose of accumulative gamma-rays irradiation can damage the activity of antioxidase. |
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