| 朱新华,刘月辉.糖皮质激素在豚鼠鼻黏膜辐射损伤中的防护作用[J].中华放射医学与防护杂志,2009,29(1):31-34 |
| 糖皮质激素在豚鼠鼻黏膜辐射损伤中的防护作用 |
| Effects of intranasal corticosteroids on radiated nasal mucosa of guinea pig |
| 投稿时间：2008-04-24 |
| DOI：10.3760/cma.j.issn.0254-5098.2009-01.009 |
| 中文关键词: 鼻黏膜 电离辐射 丙酸氟替卡松 组织病理学 细胞因子 |
| 英文关键词:Nasal mucosa Ionizing radiation Corticosteroids Histopathology Cytokine |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨鼻腔局部应用糖皮质激素[丙酸氟替卡松(FP)]在鼻黏膜辐射损伤中的防护作用机制。方法 选用健康豚鼠50只,随机均分至对照组和用药组。用医用直线加速器6 MV X线进行鼻部5 Gy照射,1次/周,共3周。照射完成后第2天用药组豚鼠鼻腔局部喷FP,分别于喷药后1周、1、2、3、4个月随机处死豚鼠各5只,光镜及电镜下观察鼻黏膜病理学变化。豚鼠处死前心脏穿刺采血,用ELISA方法检测血清细胞因子IL-1、IL-6、TNF-α的含量变化。结果 用药组豚鼠鼻黏膜早期炎性反应较对照组轻,4个月后纤毛覆盖率明显提高(72.9%与50.2%),黏膜下腺体萎缩减轻并表现出一定的分泌功能。用药组血清细胞因子IL-1升高明显且前2个月维持在高水平,2个月后开始下降,4个月时与对照组水平相当;而IL-6、TNF-α则始终降低。结论 鼻腔局部应用FP减轻了豚鼠鼻黏膜辐射损伤,而血清细胞因子IL-1、IL-6、TNF-α浓度的变化可能是其保护作用机制之一。 |
| 英文摘要: |
| Objective To investigate a mechanism protected radiation-induce injure for radiated guinea pigsnasal mucosa treated with intranasal corticosteroids(fluticasone nasal cavity spray).Methods 50 health guinea pigs were divided into 2 groups randomly: the irradiated group (control group) with 25 guinea pigs and the administration group after irradiation (test group)with 25 guinea pigs. The nasal part of all guinea pigs were performed irradiation by the 6 MV X-ray with single 5 Gy, one time each week for three weeks. The guinea pigs of test group received intranasal corticosteroids with one time every day and one spray each side nasal cavity on the second day after three weeks irradiation. Five guinea pigs in each group were sacrificed randomly at 1 week, 1 month, 2 months, 3 months and 4 months after irradiation, and the histopathologic changes were observed under optical microscope and electron microscope. At the same time, blood were drawn from the heart and the concentration of IL-1,IL-6 and TNF-α in serum were measured by ELISA.Results The early nasal mucosa inflammatory reaction of the test group was less than the control group. The coverage rate of cilia of the test group was much than that of the control group (72.9% vs 50.2%) at four months after irradiation. The atrophy of submucosal glandular organ was lessened and they displayed some extent secretory function. The concentration of IL-1 in serum was increased very much in the test group compared with the control group after irradiation and kept higher level in the first two months. After two months, it began to decrease;on four months, it still kept equivalency level with the control group. The concentration of IL-6 and TNF-α in serum were reduced all the while.Conclusion The intranasal corticosteroids with fluticasone nasal cavity spray can reduce radiation-induced injury of guinea pigs nasal mucosa. The concentration change of IL-1, IL-6 and TNF-α in serum may be one of mechanism protected radiation-induce injure of guinea pigs nasal mucosa. |
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