| 涂序珉,郭鲜花,阮健磊,雷苏文,吕慧敏,李文建,王菊芳.电离辐射致K562细胞旁效应的实验研究[J].中华放射医学与防护杂志,2009,29(1):20-22 |
| 电离辐射致K562细胞旁效应的实验研究 |
| Experimental study on bystander effects K562cells induced by ionizing radiation |
| 投稿时间：2008-06-02 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.006 |
| 中文关键词: 60Co γ线 旁效应 K562细胞 凋亡 |
| 英文关键词:60Co γ-rays Bystander effect K562 cell Apoptosis |
| 基金项目:国家自然科学基金重点课题资助项目(10335050) |
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| 中文摘要: |
| 目的 研究60Co γ线不同剂量照射条件培养液(ICM)培养K562细胞后引发的旁效应。方法 采用健康男性外周血自然杀伤(NK)细胞,观察不同剂量60Co γ线照射的条件培养液培养后引发的K562细胞对NK细胞敏感性的改变,同时用流式细胞仪检测K562细胞的凋亡情况。结果 照射组NK细胞活性显著增加,即受照肿瘤细胞的培养基显著影响了未受照肿瘤细胞的存活能力,从而NK细胞对未照射细胞的活性显著增加;其中,从0.5 Gy起即出现显著升高, 1.0 Gy继续增加,2.0和5.0 Gy出现波动,在8.5 Gy达到最高。照射组K562细胞的凋亡率比对照组显著增加(P<0.01),即受照肿瘤细胞的培养基显著影响了未受照肿瘤细胞的存活能力。在0.5 Gy处,即出现非常明显的效应。结论 对于K562细胞,ICM对细胞显示出明显损伤作用,NK细胞活性显著增加,凋亡率升高。提示存在培养基转移介导的电离辐射旁效应。 |
| 英文摘要: |
| Objective To study the bystander effects and associated mechanisms through irradiated conditioned medium(ICM).Methods Natural killer(NK) cells were obtained from peripheral blood samples. ICM irradiated with different doses of 60Co γ-rays was used for culturing K562 cell strain. The degree of injury of K562 cells by activated NK cells was observed, as well as the apoptosis frequency of K562 cell was investigated.Results Severe injury was induced in K562 cells cultured in ICM than the control (sham-irradiated) as shown by increased sensitivity to NK cells (P<0.05). The apoptosis frequency of K562 cell was increased significantly compared with the control cells(P<0.05).Conclusion The bystander effect induced by irradiation is existent. ICM can trigger the bystander effect on K562 cell strains. |
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