| 李蓉,任炯,冷言冰,刘晶,艾国平,徐辉,粟永萍,程天民.大鼠长期摄入贫铀对睾酮合成及StAR、P450scc基因表达的影响[J].中华放射医学与防护杂志,2009,29(1):13-16 |
| 大鼠长期摄入贫铀对睾酮合成及StAR、P450scc基因表达的影响 |
| Effect of long-term depleted uranium ingestion on testosterone production and the expression of StAR and P450scc mRNAs in rats |
| 投稿时间：2008-06-10 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.01.004 |
| 中文关键词: 贫铀 生殖毒性 激素 类固醇合成急性调节蛋白 细胞色素P450胆固醇侧链裂解酶 |
| 英文关键词:Depleted uranium Reproduction toxicity Hormone Steroidogenic acute regulatory protein Cytochome P450 cholesterol side chain lyase |
| 基金项目:国家自然科学基金资助项目(30670625);中国博士后科学基金资助项目(20060390680) |
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| 中文摘要: |
| 目的 研究长期摄入贫铀(DU)对雄性大鼠生殖功能改变的机制。方法 大鼠长期摄入贫铀,剂量分别为4和40 mgDU·kg－1·d－1。在 F0代20个月,F1代15个月时,测定其血中性激素含量,并用反转录多聚酶链式反应(RT-PCR)研究在睾酮(T)合成中起限速作用的类固醇合成急性调节蛋白(StAR)和细胞色素P450胆固醇侧链裂解酶(P450scc)基因表达的影响,同时设健康对照组。结果 食入组血清T的含量均低于健康对照组,最低为51.73 U/L,但黄体生成素(LH)、卵泡刺激素(FSH)含量均高于健康对照组。StAR mRNA的表达,除F1低剂量组(StAR/β-actin半定量值为1.35)上调外,其余组的表达均较健康对照组(1.035)下调;P450scc mRNA的表达在F0代低、高剂量组较健康对照组(P450scc/β-actin比值为0.313)下调,P450scc/β-actin降至0.21;在F1代低、高剂量组较健康对照组上调,P450scc/β-actin升至0.623。结论 长期摄入DU,可通过抑制StAR、P450scc mRNA的表达从而干扰T合成途径来抑制雄性的生殖作用。 |
| 英文摘要: |
| Objective To explore the effect of long-term depleted uranium (DU)ingestion on testosterone production in rats, and its involvement mechanism. Methods Male and female rats (F0 and F1 generations) were exposed to DU in food at dose of 4 and 40 mgDU·kg－1·d－1 (low and high-dose groups, respectively) for 160 days, respectively. The contents of testosterone (T), luteinizing hormone (LH), and follicle stimulating hormone (FSH) in serum were detected in 20 months of F0 generations, and 15 months of F1 generations. RT-PCR was used to analyze the levels of StAR mRNA and P450scc mRNA.Results Compared with the normal control group, the testosterone contents in exposed F0 and F1 generations increased, the lowest was 51.73 U/L, but those of LH and FSH decreased. The expression of StAR mRNA in the low-dose group of F1 generation (StAR/β-actin=1.35) was up-regulated, down-regulated for other groups. Compared with the normal control group (P450scc/β-actin=0.313),the expression of P450scc mRNA in the low- and high-dose groups of F0 generation were decreased (P450scc/β-actin=0.21), and those in the low- and high-dose groups of F1 generation were increased (P450scc/β-actin=0.623) (P≤0.01).Conclusion Long-term DU exposure inhibit the male reproduction by intervening the sexual hormone production through down-regulated the expression of StAR mRNA and P450scc mRNA. |
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