| 韦淑贞,谢学军,齐宇红,郭国祯,山顺林,李前文,张闯,杜云翔.旋转磁场对小鼠骨髓型急性放射病治疗效果的研究[J].中华放射医学与防护杂志,2008,28(6):660-664 |
| 旋转磁场对小鼠骨髓型急性放射病治疗效果的研究 |
| The hemoprotective effects of a rotary magnetic field in mice exposed to irradiation |
| 投稿时间：2007-11-27 |
| DOI： |
| 中文关键词: 旋转磁场 放射损伤 骨形态发生蛋白2/4 造血保护作用 |
| 英文关键词:Rotary magnetic field Radiation injury Bone morphogenetic protein2/4 Hemoprotection |
| 基金项目:第四军医大学军事预防医学系军事医学科研基金资助项目(XJ200604) |
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| 中文摘要: |
| 目的 研究旋转磁场对小鼠骨髓型急性放射病的治疗效果。方法 132只雄性BALB/c小鼠随机分为正常组(N),单纯磁疗组(M),单纯照射组(R)和照射磁疗组(R+M)4组,N组小鼠不作其他处理,R组和R+M组小鼠接受6.0 Gy剂量的 60Co γ 射线照射,M组及R+M组予以磁场处理30d,每天2次,每次1.5h。观察小鼠30d的存活率和存活期;分别于0、5、9、15、21、30d检测外周血细胞数;测定第9、23、30d骨髓单核细胞数(BMNC)、脾集落形成单位(CFU-S)、脾脏指数(脾体比)、骨髓细胞周期、细胞凋亡,观察股骨骨髓病理切片及检测骨形态发生蛋白(BMP2/4)的表达水平。结果 1N组和M组小鼠无死亡。R+M组与R组比较,磁疗提高了小鼠的存活率和存活期(P<0.01)。2R+M组与R组比较,磁疗提高了R+M组第15天外周血白细胞数及血红蛋白含量、第21天红细胞数、第30天血小板数(P<0.05)。3第9天R+M组CFU-S、BMNC数、细胞G2+M期比例高于R组,而总凋亡率低于R组(P<0.05)。第23天脾脏指数R+M组高于R组(P<0.05)。4骨髓病理检查显示,磁场可促进照射后骨髓造血组织结构的修复,R组骨髓早期较空虚,而R+M组骨髓细胞较充盈,随着时间的推移,两者均逐渐恢复正常;R+M组的骨髓BMP2/4表达水平亦明显高于R组。结论 旋转磁场对放射损伤小鼠骨髓造血具有明显的保护作用,能减少骨髓细胞的凋亡,其机制可能是促进放射损伤后造血细胞和造血微环境的修复。 |
| 英文摘要: |
| Objective To study the hemoprotective effects of a rotary magnetic field(RMF) to radiation-injured mice.Methods 132 male BALB/c mice were randomly divided into four groups: a normal group (N), a magnetic treatment group (M), an irradiation group(R) and an irradiation combining magnetic treatment group (R+M). Mice in the N group received no treatment. Mice in the R and R+M groups received total body irradiation with 6.0 Gy 60Co γ rays. Mice in the M and R+M groups were treated with a RMF for one and half an hour at a time,twice a day, totally for 30 days. The survival rate was observed for 30 days. On days 0, 5, 9, 15, 21, 30, the subjects peripheral blood cells were counted. On day 9, 23 and 30, the number of bone marrow nucleated cells (BMNCs), colony forming unit-spleen (CFU-S), spleen-body ratio, the cell cycle and apoptosis of bone marrow cells were measured. The pathological sectioning of the femur was performed and the expression level of bone morphogenetic proteins (BMP2/4) in the bone marrow was evaluated.Results 1No mice died in the N and M group. The RMF treatment increased the survival rate and survival days among the irradiated mice (P<0.01). 2The RMF treatment increased the number of blood cells in their peripheral blood of the R+M group. 3The number of BMNCs, CFU-S and the proportation of G2+M stage in the R+M were markedly higher than that of the R group, but the proportation of the apoptosis was lower than that of the R group on the 9th day (P<0.05). Furthermore, the spleen index in the R+M group was also higher than that of the R group on the 23rd day (P<0.05). 4RMF could improve the expression level of BMP2/4 in the radiation-injued mice.Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation and it accelerated the recovery of hematopoiesis and the hematopoietic microenvironment in mouse bone marrow. |
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