| 李翊,王海青.礁膜多糖的辐射防护及其机制的研究[J].中华放射医学与防护杂志,2008,28(6):615-617.LI Yi,WANG Hai-qing.Radiation protection and its molecular mechanism of the polysaccharide from Monostroma nitidum[J].Chin J Radiol Med Prot,2008,28(6):615-617 |
| 礁膜多糖的辐射防护及其机制的研究 |
| Radiation protection and its molecular mechanism of the polysaccharide from Monostroma nitidum |
| 投稿时间：2008-07-22 |
| DOI： |
| 中文关键词: 多糖 礁膜 辐射 外周血象 抗氧化作用 |
| 英文关键词:Polysaccharide Monostroma nitidum Radiation Peripheral blood parameters Antioxidation effect |
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| 中文摘要: |
| 目的 研究礁膜多糖对小鼠的辐射防护作用及其机制。方法 将昆明种小鼠随机分为正常对照组、照射对照组和3个礁膜多糖组(6、12和24 mg/kg),腹腔给药15d后进行一次性X射线照射,照射剂量为6.0Gy/只,照射后继续给药10d。观察小鼠30d存活率,检测礁膜多糖对小鼠外周血白细胞总数(WBC)、血小板(PLT)、红细胞数(RBC)、脾脏指数、脾脏淋巴细胞增殖能力、全血谷胱甘肽过氧化物酶(GSH-Px)活性、血清超氧化物歧化酶(SOD)活性以及脂质过氧化产物丙二醛(MDA)含量的影响。结果 礁膜多糖组小鼠30 d存活率比照射对照组明显提高,而且礁膜多糖能加速小鼠外周血WBC、PLT和RBC的恢复,显著增高抗氧化物酶GSH-Px 和SOD活性(P<0.01),降低脂质过氧化产物MDA含量,并且明显提高脾脏指数和增强脾脏T淋巴细胞的增殖能力(P<0.01)。结论 礁膜多糖对受照射小鼠有明显的辐射防护作用,其作用机制可能与其保护造血组织、提高机体免疫功能和抗氧化作用有关。 |
| 英文摘要: |
| Objective To study the radiation protection and its molecular mechanism of the polysaccharide from Monostroma nitidum. Methods The mice were randomly divided into the normal group, the model group and 3 treatment groups. Polysaccharide from Monostroma nitidum was applied to the treatment group for 15 d before irradiation with 6.0 Gy rays. 30-day survival rate was observed. The counts of peripheral white blood cell, thrombocytes and erythrocytes were measured.The activities of the antioxidant enzymes SOD and GSH-Px were assayed, and the damage to the cell membrane was studied by measuring the lipid peroxides MDA levels. The spleen index and transformation response of splenic lymphocyte were determined.Results 30 day survival rate significantly increased in the polysaccharide groups. The animal experiment showed the counts of peripheral white blood cell, thrombocytes and erythrocytes were significantly and more rapidly recovered in the polysaccharide group after irradiation. The polysaccharides markedly increased the activities of GSH-Px and SOD and decreased the levels of MDA in the blood. In the irradiated mice, the polysaccharide significantly increased the spleen index and transformation response of splenic lymphocyte when compared with irradiation mice. Conclusion The present observations suggest that the polysaccharide from Monostroma nitidum had a marked radioprotective effect on irradiated mice and that the biological response might be caused by the hematopoiesis recovery, antioxidant effect and immune activation. |
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