中华放射医学与防护杂志

任瑞美,黄广武,孙新东,等.RNA干扰HIF-1α对小鼠移植瘤放射增敏的研究[J].中华放射医学与防护杂志,2008,28(6):612-614.REN Rui-mei,HUANG Guang-wu,SUN Xin-dong,et al.Radiation enhancement effect of RNA interference for HIF-1α on the transplant tumor[J].Chin J Radiol Med Prot,2008,28(6):612-614

RNA干扰HIF-1α对小鼠移植瘤放射增敏的研究

Radiation enhancement effect of RNA interference for HIF-1α on the transplant tumor

投稿时间：2007-11-28

DOI：

中文关键词: RNA干扰 HIF-1α 移植瘤放疗增敏

英文关键词:RNA interference HIF-1α Transplant tumor Radiosensitivity

基金项目:山东省科技厅博士基金资助项目(2006BS03035)

作者	单位
任瑞美	250117 济南, 山东省肿瘤医院放疗二科
黄广武	广西医科大学
孙新东	250117 济南, 山东省肿瘤医院放疗二科
赵汉玺	250117 济南, 山东省肿瘤医院放疗二科
闫庆霞	250117 济南, 山东省肿瘤医院放疗二科

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中文摘要:

目的研究以多聚乙烯基亚胺(polyethylenimine, PEI)为载体行RNA干扰HIF-1α 对移植瘤的放射增敏作用。方法采用人肺腺癌细胞SPCA-1 裸鼠移植瘤模型,160只荷瘤鼠随机分为5组,每组32只。对照组,不做任何处理;单纯注射PEI 组,尾静脉注射以PEI (15μg),每周1次连用4周;单纯照射组,每次5Gy/次/每周,连续4周;单纯转染组,尾静脉注射以PEI (15μg)和pSUPER-HIF-1α质粒(40μg)混合物,每周1次,连用4周;照射联合转染组,尾静脉注射PEI和pSUPER-HIF-1α质粒混合物(PEI 15μg,pSUPER-HIF-1α质粒 40μg )24h后放疗,1次/周,5Gy/次,连续4周。每组内有16只小鼠于末次放疗后第3天处死,采用免疫组织化学法检测RNA干扰HIF-1α后HIF-1α的表达情况,另外16只用于观察生存时间,并每周称裸鼠的重量。结果 RNA干扰HIF-1α后,HIF-1α的表达下降,RNA干扰HIF-1α合并放疗,移植瘤的体积重量较单纯放疗、RNA干扰HIF-1α和空白对照组比较,下降明显,荷瘤鼠的生存期延长。结论体内试验显示,以PEI为载体行 RNA干扰HIF-1α有放疗增敏作用。

英文摘要:

Objective To determine and explore the radiation enhancement of RNA interference for HIF-1α on the transplant tumor using polycationic polyethyleninine (PEI), as a new kind of gene vector. Methods SPCA-1 nude mouse model was used. 160 nude mice bearing SPCA-1 were randomly divided into 4 treated groups and 1 control groups, each group had 32 mice. The expression of HIF-1α was studied by immunohistochemical method after RNA interference for HIF-1α. The differences of the volume, weight, survival time of the transplant tumor were studied among the simple radiation group, the simple RNA interference for HIF-1α group and the combination of radiation and RNA interference for HIF-1α. Results The expression of HIF-1α was decreased after RNA interference for HIF-1α. RNA interference for HIF-1α combined with radiation decreased the volume, weight of the transplant tumor, and prolonged its survival time period significantly than other methods.Conclusions RNA interference targeting HIF-1α might enhance the radiosensitivity of the transplant tumor using PEI as a new kind of gene vector in vitro.

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