| 齐雪松,吕慧敏,王春燕,张伟,郝述霞,孙全富,苏旭.云南东川非铀矿山矿工血清IL-2、IL-6、TNF-α检测及其基因多态性的初步研究[J].中华放射医学与防护杂志,2008,28(6):576-578 |
| 云南东川非铀矿山矿工血清IL-2、IL-6、TNF-α检测及其基因多态性的初步研究 |
| Preliminary study on serum levels of IL-2, IL-6 and TNF-α and their gene polymorphism in copper miners |
| 投稿时间：2008-07-31 |
| DOI： |
| 中文关键词: 非铀矿山 IL-2 IL-6 TNF-α 基因多态性 |
| 英文关键词:Copper mien IL-2 IL-6 TNF-α Gene polymorphism |
| 基金项目:科技部科研院所社会公益研究专项(2005DIB1J087) |
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| 中文摘要: |
| 目的 通过对云南非铀矿山矿工血清IL-2、IL-6和TNF-α浓度及基因多态性位点的检测,对氡致非铀矿山矿工机体生物效应进行初步探讨。方法 血清中IL-2、IL-6和TNF-α的浓度测定采用双抗夹心ABC-ELISA法。TNF-α(-308,G→A)检测基因型采用基因体外扩增限制性片段长度多态性分析方法,IL-2(-330, T→G)基因型检测采用PCR体外扩增后测序的方法。结果 矿工组与对照组比较,年龄(F＝1.07,P>0.05),工龄(F＝0.40,P>0.05),血清IL-2浓度(F＝0.71,P>0.05),血清IL-6浓度(F＝1.09,P>0.05),血清TNF-α浓度(F＝0.95,P>0.05),IL-2基因型频度(χ2=0.02,P>0.05)和TNF-α基因型频度(χ2=2.21,P>0.05),差异均无统计学意义。结论 云南东川非铀矿山矿工血清IL-2、IL-6和TNF-α平均浓度有下降的趋势, TNF-α(-308,A A)基因型频度有增高的趋势。 |
| 英文摘要: |
| Objective To study biologic effect of high radon exposure in non-uranium miners by measurements of serum levels of IL-2, IL-6 and TNF-α and study on their gene polymorphism.Methods Serum levels of IL-2, IL-6 and TNF-α in the miners selected from a Yunnan-based copper mine were measured by ABC-ELISA. TNF-α (-308,G→A) genotypes were identified by RFLP-PCR, IL-2 (-330, T→G) genotypes by sequence analysis.Results Compared the miners with its control group, there were no statistical significance of the concentrations of serum IL-2 (F=0.71, P>0.05), IL-6 (F=1.09, P>0.05) and TNF-α (F=0.95, P>0.05). Frequencies of IL-2 (-330, T→G) genotypes (χ2=0.02, P>0.05) and TNF-α (-308, G→A) genotype (χ2=2.21, P>0.05) were shown no statistical significance too.Conclusions Compared with the control group, concentrations of serum IL-2, IL-6 and TNF-α in miners working in the copper mine was lower, frequencies of genotypes of TNF-α (-308,A A) was higher in the miners. But all the differences were not statistical significant. |
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