| 龙清云,周云峰,胡金香,周军,乐涛.当归对兔VX2肝癌放疗联合介入治疗后肝纤维化的影响[J].中华放射医学与防护杂志,2008,28(5):514-516,538 |
| 当归对兔VX2肝癌放疗联合介入治疗后肝纤维化的影响 |
| Effect of Angelica Sinensis on liver fibrosis after radiotherapy combined with transarterial chemoembolization for rabbits with VX 2 tumor |
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| DOI： |
| 中文关键词: 兔 肝纤维化 化疗栓塞 放疗 当归注射液 |
| 英文关键词:Rabbit Hepatic fibrosis Chemoembolization Radiotherapy Angelica Sinensis injection |
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| 摘要点击次数: 2855 |
| 全文下载次数: 1908 |
| 中文摘要: |
| 目的 观察当归对兔VX2肝癌放疗(RT)联合动脉化疗栓塞(TACE)术后肝纤维化的预防作用,并探讨其预防肝纤维化的作用机制。方法 将50只新西兰白兔随机表法分为对照组(n=10)、模型组(n=10)和预防组(n=30)。模型组:兔VX2肝癌种植2周后经胃十二指肠动脉注入平阳霉素1 mg+碘油0.2 ml, 1周后单次行左肝照射20 Gy;预防组:荷VX2肝癌兔在行RT联合TACE(剂量同模型组)术时经耳静脉注射当归注射液,根据当归注射液量的不同进一步分为小剂量组(4 ml/kg)、中剂量组(6 ml/kg)和大剂量组(8 ml/kg),每周2次,共4周;对照组:不进行VX2肝癌种植,经胃十二指肠动脉注入生理盐水2 ml。于RT联合TACE术后第6周末检测HA、LN、PCⅢ和CIV,并进行肝组织HE、VG和TGF-β1免疫组织化学染色。结果 模型组的肝纤维化病理分期主要处于肝纤维化Ⅱ期和Ⅲ期,预防组的肝纤维化分期主要处于肝纤维化Ⅰ期,各组之间分期差异有统计学意义,肝纤维化分期与当归治疗相关(r=0.7631,P<0.01);模型组血清HA、LN、PCⅢ和CIV明显高于对照组(P<0.01),预防组与模型组相比明显降低(P<0.05);模型组肝组织TGF-β1表达增强,预防组TGF-β1表达较模型组明显减少,且不同剂量当归预防组之间存在量效关系(r=0.4427,P<0.01)。结论 当归对兔VX2肝癌RT联合TACE术后肝纤维化有预防作用,TGF-β1参与其调控机制。 |
| 英文摘要: |
| Objective To evaluate the effect of Angelica Sinensis on liver fibrosis after radiotherapy(RT) combined with transarterial chemoembolization(TACE) for rabbits with VX 2 tumor, and the mechanism of anti-hepatic fibrosis. Methods Fifty New Zealand white rabbits were divided randomly into three groups:Control group(n=10) was given saline 2 ml by gastroduodenal artery, model group (n=10) was given pingyangmycin 1 mg and iodized oil 0.2 ml, and exposed to single fraction of 20 Gy, Angelica group(n=30) undergoing RT combined with TACE was given Angelica Sinensis injection. According to different doses of Angelica Sinensis, Angelica group was divided into low-dose group (4 ml/kg), middle dose group (6 ml/kg) and high-dose group (8 ml/kg), twice per week for 4 weeks.Serum levels of HA, LN, PCⅢ, CIV and liver specimens were obtained at the end of 6th week after RT combined with TACE.The histological changes were determined by HE,VG staining and TGF-β1 immunohistochemistry staining. Results Stages of liver fibrosis in the model group were mostly stages Ⅱ and Ⅲ, and stages of liver fibrosis in the Angelica group were mostly stages I,there was significant difference between the model group and the Angelica group, and stages of liver fibrosis were associated with Angelica intervention treatment (r=0.7631, P<0.01). Serum HA, LN, PCⅢ and CIV in the model group were higher than those in the control group(P<0.01), TGF-β1 expression in the Angelica group was significantly reduced compared with the wodel group and different doses of Angelica group showed dose-effect (r=0.4427, P<0.01). Conclusions Angelica Sinensis injection has inhibitive effect on liver fibrosis after RT combined with TACE for rabbits with VX 2 tumor,the mechanism of anti-hepatic fibrosis might possibly be associated with down-regulating TGF-β1. |
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