| 谭永红,向德兵,史曦凯,等.pcDNA3.1+Ape1质粒转染对人脐静脉内皮细胞辐射敏感性的影响[J].中华放射医学与防护杂志,2008,28(5):492-494.TAN Yong-hong,XIANG De-ping,SHI Xi-kai,et al.Effect on radiosensitivity in human umbilical vein endothelial cells after transfection of pcDNA3.1+Ape1 plasmid[J].Chin J Radiol Med Prot,2008,28(5):492-494 |
| pcDNA3.1+Ape1质粒转染对人脐静脉内皮细胞辐射敏感性的影响 |
| Effect on radiosensitivity in human umbilical vein endothelial cells after transfection of pcDNA3.1+Ape1 plasmid |
| 投稿时间：2007-08-13 |
| DOI： |
| 中文关键词: Ape1/Ref-1 内皮细胞 辐射敏感性 |
| 英文关键词:Ape1/Ref-1 Endothelial cell Radiosensitivity |
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| 中文摘要: |
| 目的 探讨pcDNA3.1+Ape1质粒转染对血管内皮细胞电离辐射敏感性的影响。方法 以pcDNA3.1+空载体转染、未转染细胞作对照,在细胞转染pcDNA3.1+Ape1质粒后48 h,给予3组细胞2、4、6和8 Gy高能X线照射,采用碱性单细胞凝胶电泳和克隆形成实验,分析在不同辐射剂量条件下pcDNA3.1+Ape1质粒转染对人脐静脉内皮细胞辐射敏感性的影响。结果 与未转染细胞相比,3 μg pcDNA3.1+Ape1质粒转染细胞的初始OTM和残存OTM值在2 Gy照射时显著降低(P<0.01),但8Gy照射时差异无统计学意义(P>0.05);SF2显著增高(P<0.05),但SF4、SF6及SF8的差异均无统计学意义(P>0.05)。结论 pcDNA3.1+Ape1质粒转染可增加内皮细胞对低剂量电离辐射的抵抗能力。 |
| 英文摘要: |
| Objective To investigate the possible effects on radiosensitivity in human umbilical vein endothelial cells after transfection of pcDNA3.1+Ape1 plasmid. Methods The expressing vector pcDNA3.1+Ape1, the control vector pcDNA3.1+ or non-transfection cells was irradiated by 2, 4, 6, and 8 Gy photon beam at 48h post-transfection. The value of initial and residual Oliver tail moment (OTM) under the alkaline single cell gelelectrophoresis assay and the colony forming test were utilized as the markers for the evaluaton of cells intrinsic radiosensitivity. The effect on radiosensitivity in human umbilical vein endothelial cells after transfection of the expressing vector pcDNA3.1+Ape1 was analyzed according to the radio-dose, compared to the empty vecor control and non-transfection cells. Results The initial and residual OTM value of endothelial cells transfected by 3 μg pcDNA3.1+Ape1 plasmid was lower significantly than ones of endothelial cells untransfected at 2 Gy irradiation (P<0.01), but was no significant difference at 8Gy (P>0.05), and SF2 was higher remarkably in transfected cells than one in untransfected cells (P<0.05), but SF4, SF6 and SF8 were no significant differences (all of P>0.05). Conclusions The transfection of pcDNA3.1+Ape1 plasmid could enhance radioresistance of endothelial cells to the low-dose irradiation. |
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