| 蒋定文,雷呈祥,沈先荣,马丽,杨翊方,彭武林,戴寿荣.低剂量率裂变中子照射对大鼠外周血淋巴细胞亚群的影响[J].中华放射医学与防护杂志,2008,28(5):488-491 |
| 低剂量率裂变中子照射对大鼠外周血淋巴细胞亚群的影响 |
| Effects of low dose rate fission neutron irradiation on the lymphocyte subpopulations of peripheral blood in rats\ |
| 投稿时间：2007-08-29 |
| DOI： |
| 中文关键词: 裂变中子 低剂量率 淋巴细胞亚群 大鼠 |
| 英文关键词:Fission neutron Low dose rate Lymphocyte subpopulations Rat |
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| 中文摘要: |
| 目的 探讨低剂量率中子长期照射对大鼠外周血细胞亚群的影响。方法 96只雄性大鼠分为对照组和照射组,照射组每天用低剂量率中子252Cf(吸收剂量率为0.35 mGy/h)照射20.5h,在照射的第14、28、42、56和70天(累积剂量分别为0.1、0.2、0.3、0.4和0.5 Gy)及停止照射后第35天各取8只大鼠,用血细胞计数仪检测大鼠外周血WBC、用流式细胞仪检测外周血CD4+CD3+、 CD8+CD3+、CD45RA+/CD161α+亚群的变化。结果 累积剂量为0.3、0.4及0.5 Gy时WBC明显低于对照组(P<0.05),停止照射后35 d,WBC显著低于对照组(P<0.01);累积剂量为0.1、0.3、0.4、0.5 Gy及停止照射后35 d,外周血CD4+CD3-细胞比例显著高于对照组(P<0.01或<0.05);累积剂量为0.2和0.3 Gy时CD8+CD3-细胞比例显著高于对照组(P<0.05或P<0.01)。而累积剂量为0.1 Gy时的CD4+CD3+细胞比例及0.1和0.2 Gy时的CD8+CD3+细胞比例明显高于同一天对照组(P<0.01或<0.05)。另外,低剂量率中子长期照射可使累积剂量为0.2~0.3 Gy的外周血NK细胞(CD161α+ CD45RA-)显著升高,累积剂量为0.1~0.5 Gy及停止照射后35 d照射组的外周血B细胞(CD161α- CD45RA+)比例明显下降。结论 低剂量率裂变中子长期照射可使外周血淋巴细胞TCR基因突变,使大鼠外周血WBC减少,淋巴细胞中B细胞减少,NK细胞细胞比例升高,这种变化在停止照射后一段时间仍可能难以恢复。 |
| 英文摘要: |
| Objective To evaluate the effects of long-term, low dose rate fission neutron irradiation on lymphocyte subpopulations in peripheral blood of rats. Methods Ninety-six rats were randomly divided into control group and irradiated group exposed to low dose rate fission neutron (252Cf,0.35 mGy/h) for 20.5 h every day.At days 14,28,42,56 and 70 d after irradiation and 35 d after stopping irradiation, After 8 rats of each group were killed, WBC and lymphocyte subpopulations of CD4+CD3+,CD8+CD3+and CD45RA+/CD161α+ in peripheral blood were estimated respectively. Results Compared with the control group,WBC was reduced significantly at dose of 0.3, 0.4 and 0.5Gy (P<0.05), and was decreased remarkably at 35 d after stopping irradiation(P<0.01). The rate of CD4+CD3- was evidently higher compared with control group at doses of 0.1,0.3, 0.4 and 0.5 Gy and 35 d after stopping irradiation (P<0.01, P<0.01 and P<0.05), and the rate of CD8+CD3- was obviously higher compared with control group at dose of 0.2 and 0.3 Gy (P<0.05 and P<0.01). On the other hand, the rate of CD4+CD3+ at dose of 0.1 Gy (P<0.01) and the rate of CD8+CD3+ at doses of 0.1 and 0.2 Gy (P<0.05) were higher when compared with control group. At the same time,peripheral blood NK cells(CD161α+ CD45RA-) was increased significantly at doses of 0.2-0.3 Gy, and peripheral blood B cells(CD161α- CD45RA+) was reduced remarkably at doses of 0.1-0.5 Gy and 35 d after stopping irradiation compared with the control group. Conclusions Long-term irradiation with low dose rate fission neutron could make TCR (T-cell-receptor) mutant, therefore, WBC, B cells in peripheral blood significantly reduced and NK cells increased. These changes may could not recover at 35 d after stopping irradiation. |
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