| 潘燕,袁德晓,邵春林.DNA损伤修复在镉诱导的细胞辐射适应性中的作用[J].中华放射医学与防护杂志,2008,28(5):466-470.PAN Yan,YUAN De-xiao,SHAO Chun-lin.Role of DNA repair capacity in cadmium induced cellular adaptive response[J].Chin J Radiol Med Prot,2008,28(5):466-470 |
| DNA损伤修复在镉诱导的细胞辐射适应性中的作用 |
| Role of DNA repair capacity in cadmium induced cellular adaptive response |
| 投稿时间：2008-05-08 |
| DOI： |
| 中文关键词: CdCl2 适应性反应 γ辐射 DNA修复 |
| 英文关键词:CdCl2 Adaptive response γ-irradiation DNA repair |
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| 中文摘要: |
| 目的 探讨不同的修复能力在镉诱导的细胞适应性反应中的作用,并对PI3K家族参与细胞适应性信号传导的作用做初步探索。方法 0.1 或1 μmol/LCdCl2预处理细胞后,间隔2 h,再对细胞进行50、100 μmol/L CdCl2或1、2 Gy辐射处理,微核法检测细胞表达适应性反应程度。另外,在预处理和间隔时间中,用20 μmol/L wortmannin处理细胞。结果 低浓度能够诱导细胞对随后50 μmol/L CdCl2或1 Gy γ射线照射产生适应性反应。当攻击剂量为50 μmol/L CdCl2,EM-C11细胞的适应反应程度低于另外两株细胞。三种细胞的适应性反应均可被wortmannin抑制。结论 细胞适应性程度的大小与其DNA损伤的修复能力、预处理和攻击处理剂量相关,相对于DSB,SSB在诱导细胞表达适应性反应中起主要作用。ATM激酶参与细胞适应性信号通路的信号传导。 |
| 英文摘要: |
| Objective To investigate cadmium induced adaptive responses (AR) to either toxicant challenge or irradiation and also the role of PI3K family in the AR. Methods Cells were pre-treated with 0.1 or 1 μmol/L cadmium and then challenged by 50, 100 μmol/L cadmium or 1, 2 Gy γ-rays irradiation. Micronucleus induction was measured to evaluate the magnitude of AR. In some experiments, cells were treated with wortmannin during and after pretreatment. Results Cadmium of sub-lethal concentration could induce AR in all the cells toward 50 μmol/L cadmium or 1 Gy irradiation. When challenged by 50 μmol/L CdCl2, EM-C11 cells had an AR less apparent than the other two cell lines. Moreover, treatment of cells with wortmannin eliminated the AR in all three cell lines. Conclusions The magnitudes of AR in adapted cells may be related to multiple factors, such as DNA repair capacity, the priming and challenging dose of cadmium or irradiation. SSB rather than DSB repair is mainly involved in the cadmium induced AR and this cellular response may be mediated through ATM pathway. |
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